SMARCAD1 Antibody - #DF2598
Product: | SMARCAD1 Antibody |
Catalog: | DF2598 |
Description: | Rabbit polyclonal antibody to SMARCAD1 |
Application: | WB IHC |
Reactivity: | Human |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 117 kDa; 117kD(Calculated). |
Uniprot: | Q9H4L7 |
RRID: | AB_2839804 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2598, RRID:AB_2839804.
Fold/Unfold
ATP dependent helicase 1; ATP-dependent helicase 1; ETL1; hHEL1; Smarcad1; SMRCD_HUMAN; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1;
Immunogens
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level) (PubMed:29409814).
- Q9H4L7 SMRCD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDITEKTEDSSVPETPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTVIIVSEPSEDEESQGLPTMARRNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKLIESTSTMDGAIAAALLMFGDAGGGPRKRKLSSSSEPYEEDEFNDDQSIKKTRLDHGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFNPKRVVEDSEYDSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRPFNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSILNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNWLREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAHAKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRKMANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHINNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H4L7 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
S33 | Phosphorylation | Uniprot | |
S34 | Phosphorylation | Uniprot | |
S37 | Phosphorylation | Uniprot | |
S39 | Phosphorylation | Uniprot | |
S50 | Phosphorylation | Uniprot | |
T54 | Phosphorylation | Uniprot | |
S57 | Phosphorylation | Uniprot | |
T60 | Phosphorylation | Uniprot | |
T63 | Phosphorylation | Uniprot | |
S66 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | Uniprot | |
T71 | Phosphorylation | Uniprot | |
K77 | Sumoylation | Uniprot | |
K77 | Ubiquitination | Uniprot | |
S79 | Phosphorylation | Uniprot | |
Y82 | Phosphorylation | Uniprot | |
K84 | Sumoylation | Uniprot | |
S95 | Phosphorylation | Uniprot | |
S96 | Phosphorylation | Uniprot | |
S98 | Phosphorylation | Uniprot | |
S103 | Phosphorylation | Uniprot | |
T114 | Phosphorylation | Uniprot | |
T119 | Phosphorylation | Uniprot | |
S124 | Phosphorylation | Uniprot | |
S127 | Phosphorylation | Uniprot | |
S132 | Phosphorylation | Uniprot | |
T137 | Phosphorylation | Uniprot | |
S146 | Phosphorylation | Uniprot | |
S152 | Phosphorylation | Uniprot | |
K158 | Ubiquitination | Uniprot | |
K161 | Sumoylation | Uniprot | |
K161 | Ubiquitination | Uniprot | |
S173 | Phosphorylation | Uniprot | |
K209 | Ubiquitination | Uniprot | |
S211 | Phosphorylation | Uniprot | |
S212 | Phosphorylation | Uniprot | |
S213 | Phosphorylation | Uniprot | |
S214 | Phosphorylation | Uniprot | |
Y217 | Phosphorylation | Uniprot | |
S239 | Phosphorylation | Uniprot | |
S242 | Phosphorylation | Uniprot | |
S245 | Phosphorylation | Uniprot | |
S254 | Phosphorylation | Uniprot | |
K258 | Sumoylation | Uniprot | |
K258 | Ubiquitination | Uniprot | |
K261 | Ubiquitination | Uniprot | |
K268 | Methylation | Uniprot | |
K268 | Ubiquitination | Uniprot | |
K276 | Ubiquitination | Uniprot | |
S302 | Phosphorylation | Uniprot | |
S304 | Phosphorylation | Uniprot | |
K310 | Ubiquitination | Uniprot | |
S313 | Phosphorylation | Uniprot | |
S314 | Phosphorylation | Uniprot | |
S316 | Phosphorylation | Uniprot | |
Y319 | Phosphorylation | Uniprot | |
K335 | Sumoylation | Uniprot | |
K346 | Sumoylation | Uniprot | |
K346 | Ubiquitination | Uniprot | |
K352 | Acetylation | Uniprot | |
K388 | Ubiquitination | Uniprot | |
S397 | Phosphorylation | Uniprot | |
K410 | Ubiquitination | Uniprot | |
S423 | Phosphorylation | Uniprot | |
K430 | Ubiquitination | Uniprot | |
K446 | Ubiquitination | Uniprot | |
K461 | Ubiquitination | Uniprot | |
K468 | Ubiquitination | Uniprot | |
K695 | Sumoylation | Uniprot | |
K695 | Ubiquitination | Uniprot | |
K705 | Ubiquitination | Uniprot | |
K712 | Ubiquitination | Uniprot | |
K716 | Sumoylation | Uniprot | |
K724 | Sumoylation | Uniprot | |
K724 | Ubiquitination | Uniprot | |
K729 | Acetylation | Uniprot | |
K760 | Ubiquitination | Uniprot | |
K767 | Ubiquitination | Uniprot | |
K780 | Ubiquitination | Uniprot | |
K796 | Ubiquitination | Uniprot | |
K798 | Ubiquitination | Uniprot | |
S801 | Phosphorylation | Uniprot | |
S853 | Phosphorylation | Uniprot | |
T880 | Phosphorylation | Uniprot | |
Y897 | Phosphorylation | Uniprot | |
K903 | Ubiquitination | Uniprot | |
K970 | Ubiquitination | Uniprot | |
K989 | Ubiquitination | Uniprot | |
K994 | Acetylation | Uniprot |
Research Backgrounds
DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.
Nucleus. Chromosome.
Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage.
Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).
Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA.
Belongs to the SNF2/RAD54 helicase family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
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