Product: SYCP3 Antibody
Catalog: DF2579
Description: Rabbit polyclonal antibody to SYCP3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Chicken
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: Q8IZU3
RRID: AB_2839785

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:20-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
SYCP3 Antibody detects endogenous levels of total SYCP3.
RRID:
AB_2839785
Cite Format: Affinity Biosciences Cat# DF2579, RRID:AB_2839785.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

choline phosphotransferase 1; chpt1; COR 1; COR1; MGC71888; RNASCP3; SCP 3; SCP-3; SCP3; SPGF4; Sycp 3; Sycp3; SYCP3_HUMAN; Synaptonemal complex protein 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8IZU3 SYCP3_HUMAN:

Testis-specific.

Description:
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
Sequence:
MVSSGKKYSRKSGKPSVEDQFTRAYDFETEDKKDLSGSEEDVIEGKTAVIEKRRKKRSSAGVVEDMGGEVQNMLEGVGVDINKALLAKRKRLEMYTKASLKTSNQKIEHVWKTQQDQRQKLNQEYSQQFLTLFQQWDLDMQKAEEQEEKILNMFRQQQKILQQSRIVQSQRLKTIKQLYEQFIKSMEELEKNHDNLLTGAQNEFKKEMAMLQKKIMMETQQQEIASVRKSLQSMLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Chicken
80
Zebrafish
70
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZU3 As Substrate

Site PTM Type Enzyme
K46 Acetylation
K52 Acetylation
S58 Phosphorylation
K106 Ubiquitination

Research Backgrounds

Function:

Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility. Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity).

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere.
Note: It is present in early unpaired cores, in the lateral domains of the synaptonemal complex and in the chromosome cores when they separate at diplotene. It is found axial to the metaphase I chromosomes and in association with pairs of sister centromeres. The centromere-associated protein becomes dissociated from the centromeres at anaphase II and is not found in mitotic metaphase centromeres.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis-specific.

Subunit Structure:

Component of the lateral elements of synaptonemal complexes (By similarity). Homotetramer; the tetrameric helix bundles assemble end to end into long homopolimeric fibers that exhibit a transversal striation with a periodicity of about 20 nm (in vitro). Interacts with SYCP2 (By similarity). Forms a complex with EWSR1, PRDM9, REC8 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).

Family&Domains:

Composed of a long central coiled coil domain. The N-terminal and C-terminal regions interact with DNA.

Belongs to the XLR/SYCP3 family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

References

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=8.5]

Application: WB    Species: Rat    Sample: testicular tissues

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p‐Akt, caspase‐9, Bcl‐2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3. (B) Statistical analysis of band intensity by Student's t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. NS, not significant; Sham, sham group; Surgical treatment, surgical treatment group; VC, varicocele group

Application: WB    Species: rat    Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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