MAP3K12 Antibody - #DF2334
Product: | MAP3K12 Antibody |
Catalog: | DF2334 |
Description: | Rabbit polyclonal antibody to MAP3K12 |
Application: | WB IHC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 95 kDa; 93kD(Calculated). |
Uniprot: | Q12852 |
RRID: | AB_2839558 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2334, RRID:AB_2839558.
Fold/Unfold
Dlk; Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; Leucine-zipper protein kinase; M3K12_HUMAN; Map3k12; MAPK-upstream kinase; MEKK12; Mitogen-activated protein kinase kinase kinase 12; Mixed lineage kinase; MUK; Protein kinase MUK; ZPK; ZPKP1;
Immunogens
- Q12852 M3K12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTHVLQLHEQDAGGPGGAAGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYSTEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKERELLRREQALERRCPGLLKPHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGLPGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRTAVPPHEPGGPGSPGGLGGGPSAWEACPPALRGLHHDLLLRKMSSSSPDLLSAALGSRGRGATGGAGDPGSPPPARGDTPPSEGSAPGSTSPDSPGGAKGEPPPPVGPGEGVGLLGTGREGTSGRGGSRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELTSSQRWPQSLNMRQSLSTFSSENPSDGEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDPPPSEVIPGPEPSSLPIPHQELLRERGPPNSEDSDCDSTELDNSNSVDALRPPASLPP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q12852 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T9 | Phosphorylation | Uniprot | |
S11 | Phosphorylation | Uniprot | |
T43 | Phosphorylation | Uniprot | |
S477 | Phosphorylation | Uniprot | |
S500 | Phosphorylation | Uniprot | |
S536 | Phosphorylation | Uniprot | |
S551 | Phosphorylation | Uniprot | |
S555 | Phosphorylation | Uniprot | |
S609 | Phosphorylation | Uniprot | |
S615 | Phosphorylation | Uniprot | |
S634 | Phosphorylation | Uniprot | |
T642 | Phosphorylation | Uniprot | |
S645 | Phosphorylation | Uniprot | |
S695 | Phosphorylation | Uniprot | |
T699 | Phosphorylation | Uniprot | |
S757 | Phosphorylation | Uniprot |
PTMs - Q12852 As Enzyme
Research Backgrounds
Part of a non-canonical MAPK signaling pathway. Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1. May be an activator of the JNK/SAPK pathway.
Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
The activity of MAP3K12 can be regulated through its proteasomal degradation. APOE, through a receptor-mediated mechanism, activates MAP3K12 by preventing its proteasomal degradation.
Cytoplasm. Cell membrane.
Note: Behaves essentially as an integral membrane protein.
Highly expressed in brain and kidney.
Homodimer (By similarity). Interacts with MBIP.
Interacts with MBIP through the leucine-zipper motif.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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