Product: Tubulin gamma Antibody
Catalog: AF7012
Description: Rabbit polyclonal antibody to Tubulin gamma
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Rabbit, Xenopus
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: P23258
RRID: AB_2839419

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 50ul $150 In stock
 100ul $250 In stock
 200ul $350 In stock
 1ml $1200 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:000-1:5000, IHC 1:50-1:200, IF/ICC 1:200-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(87%), Rabbit(87%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Tubulin gamma Antibody detects endogenous levels of total Tubulin gamma.
RRID:
AB_2839419
Cite Format: Affinity Biosciences Cat# AF7012, RRID:AB_2839419.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

TUBG1, Gamma-1-tubulin, GCP-1, Gamma Tubulin, TUBGCP1, Tubulin gamma chain, TUBG, Tubulin, gamma polypeptide, Tubulin gamma-1 chain, Tubulin, gamma 1

Immunogens

Immunogen:

A synthesized peptide derived from human Tubulin gamma.

Uniprot:
Gene(ID):
Description:
TUBG1 Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2;
Sequence:
MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
87
Zebrafish
87
Xenopus
80
Pig
73
Horse
73
Bovine
73
Sheep
73
Dog
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23258 As Substrate

Site PTM Type Enzyme
S32 Phosphorylation
K48 Ubiquitination
S80 Phosphorylation P24941 (CDK2)
K84 Ubiquitination
K113 Ubiquitination
S129 Phosphorylation
S131 Phosphorylation Q8TDC3-2 (BRSK1)
K164 Ubiquitination
K193 Ubiquitination
S284 Phosphorylation
K287 Ubiquitination
R295 Methylation
K301 Ubiquitination
S306 Phosphorylation
T307 Phosphorylation
K335 Ubiquitination
S336 Phosphorylation
K344 Ubiquitination
K363 Ubiquitination
S364 Phosphorylation
S385 Phosphorylation Q8TDC3 (BRSK1)
K397 Acetylation
K400 Acetylation
K400 Ubiquitination
K410 Ubiquitination
K415 Ubiquitination
T423 Phosphorylation
S424 Phosphorylation

Research Backgrounds

Function:

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.

PTMs:

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Interacts with SAS6 and NUP62 at the centrosome.

Family&Domains:

Belongs to the tubulin family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

References

1). Entrectinib ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β1 signaling pathway. International Immunopharmacology, 2022 (PubMed: 36375321) [IF=5.6]

2). Ficus hirta Vahl. ameliorates liver fibrosis by triggering hepatic stellate cell ferroptosis through GSH/GPX4 pathway. Journal of ethnopharmacology, 2024 (PubMed: 39009327) [IF=5.4]

Application: WB    Species: Mouse    Sample:

Fig. 3. The effect of FV on MCD-induced hepatic fibrosis in mice. (A)H&E staining of liver tissues in different groups; (B)Masson image staining of liver tissues in different groups; (C)Sirus Red staining of liver tissues in different groups; (D)liver index; (E)quantification of the collagen fiber area (%) using Image J; (F)quantification of the collagen fiber area (%), using Image J; (G-J)serum ALT & AST, liver TC & TG (n = 6); (K-M)The fibrosis protein levels of α-SMA, collagen Ⅰ were detected by western blotting and the relative ratios were calculated (n = 3); (N–R) The relative mRNA expression levels of α-SMA, COL1A1, TGF-β1, TNF-α and IL-1β were determined by qRT -PCR (n = 3). NC, normal group; MCD, MCD diet group; FV-L, FV-L group; FV-H, FV-L group. The data are expressed in the format of mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

3). Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35592528)

Application: WB    Species: Mouse    Sample:

Figure 4 Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca, Cd36, Cpt1α, Srebp-1, Hmgcr, Pparα, Pparγ, and Scd1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1α, and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1β, and TNF-α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1β, TNF-α, and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1β, IL-6, and TNF-α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. #P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

4). The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells. Aging (Albany NY), 2023 (PubMed: 38019477)

Application: WB    Species: Human    Sample: HNPCs

Figure 1 Glycitin alleviated the TNF-α-induced inflammatory response. (A, B) Transcriptional and (C) protein levels of iNOS and COX-2 in human primary nucleus pulposus cells as determined by RT-PCR and Western blotting. (D, E) Quantitative analysis of immunoblotting in (C), assayed by ImageJ program. (F, G) IF staining of COX-2, fluorescence intensity analysis was performed using ImageJ program. Scale bar: 100 μm. The values represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.

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