Product: SLC5A1 Antibody
Catalog: DF7202
Description: Rabbit polyclonal antibody to SLC5A1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60kDa; 73kD(Calculated).
Uniprot: P13866
RRID: AB_2839154

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
SLC5A1 Antibody detects endogenous levels of total SLC5A1.
RRID:
AB_2839154
Cite Format: Affinity Biosciences Cat# DF7202, RRID:AB_2839154.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D22S675; High affinity sodium glucose cotransporter 1; High affinity sodium glucose cotransporter; High affinity sodium-glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; Na+/glucose cotransporter 1; NAGT; SC5A1_HUMAN; SGLT 1; SGLT1; SLC5A1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5 (sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P13866 SC5A1_HUMAN:

Expressed mainly in intestine and kidney.

Description:
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
92
Dog
92
Chicken
92
Horse
83
Zebrafish
83
Rabbit
83
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13866 As Substrate

Site PTM Type Enzyme
Y236 Phosphorylation
T242 Phosphorylation
N248 N-Glycosylation
T271 Phosphorylation
S335 Phosphorylation
S418 Phosphorylation
S437 Phosphorylation
T587 Phosphorylation
Y606 Phosphorylation

Research Backgrounds

Function:

Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

PTMs:

N-glycosylation is not necessary for the cotransporter function.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed mainly in intestine and kidney.

Family&Domains:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Research Fields

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Xinbao Pill ameliorates heart failure via regulating the SGLT1/AMPK/PPARα axis to improve myocardial fatty acid energy metabolism. Chinese medicine, 2024 (PubMed: 38862959) [IF=4.9]

2). Phlorizin Exerts Direct Protective Effects on Palmitic Acid (PA)-Induced Endothelial Dysfunction by Activating the PI3K/AKT/eNOS Signaling Pathway and Increasing the Levels of Nitric Oxide (NO). Medical Science Monitor Basic Research, 2018 (PubMed: 29307883) [IF=2.8]

Application: WB    Species: human    Sample: HUVECs

Figure 1.| PA stimulates expression of SGLT1 and SGLT2 in HUVECs. Cells were incubated for 18 h in the presence of PA (300 µM). (A, B) SGLT1 and SGLT2 proteins in the samples were analyzed by Western blotting. Bar plots show the summarized data of the relative density after being normalized to GAPDH and are expressed as average percentage of control ±SEM. (C, D) Quantitative PCR analysis of mRNA expression of SGLT1 and SGLT2, normalized to GAPDH mRNA. ** P<0.01.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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