Product: Leptin Receptor Antibody
Catalog: DF7139
Description: Rabbit polyclonal antibody to Leptin Receptor
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit, Chicken
Mol.Wt.: 96kDa; 132kD(Calculated).
Uniprot: P48357
RRID: AB_2839093

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Product Info

Source:
Rabbit
Application:
WB 1:500–1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Rabbit(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
Leptin Receptor Antibody detects endogenous levels of total Leptin Receptor.
RRID:
AB_2839093
Cite Format: Affinity Biosciences Cat# DF7139, RRID:AB_2839093.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEP-R; LEPR; LEPR_HUMAN; LEPRD; Leptin receptor; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; Leptin receptor precursor; OB R gene related protein; OB receptor; OB-R; OB-RGRP; obl; Obr;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P48357 LEPR_HUMAN:

Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218). Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075).

Description:
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).
Sequence:
MICQKFCVVLLHWEFIYVITAFNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDAGLYVIVPVIISSSILLLGTLLISHQRMKKLFWEDVPNPKNCSWAQGLNFQKPETFEHLFIKHTASVTCGPLLLEPETISEDISVDTSWKNKDEMMPTTVVSLLSTTDLEKGSVCISDQFNSVNFSEAEGTEVTYEDESQRQPFVKYATLISNSKPSETGEEQGLINSSVTKCFSSKNSPLKDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYLGVTSIKKRESGVLLTDKSRVSCPFPAPCLFTDIRVLQDSCSHFVENNINLGTSSKKTFASYMPQFQTCSTQTHKIMENKMCDLTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Chicken
83
Zebrafish
57
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48357 As Substrate

Site PTM Type Enzyme
N23 N-Glycosylation
S36 Phosphorylation
N41 N-Glycosylation
S42 Phosphorylation
N56 N-Glycosylation
N73 N-Glycosylation
N81 N-Glycosylation
N98 N-Glycosylation
S172 Phosphorylation
N187 N-Glycosylation
N206 N-Glycosylation
T208 Phosphorylation
N276 N-Glycosylation
N347 N-Glycosylation
N397 N-Glycosylation
N516 N-Glycosylation
Y621 Phosphorylation
S623 Phosphorylation
N624 N-Glycosylation
S626 Phosphorylation
K658 Ubiquitination
N659 N-Glycosylation
N688 N-Glycosylation
N697 N-Glycosylation
N728 N-Glycosylation
N750 N-Glycosylation
Y795 Phosphorylation
Y796 Phosphorylation
S882 Phosphorylation
T937 Phosphorylation
S944 Phosphorylation
T946 Phosphorylation
Y986 Phosphorylation
Y1079 Phosphorylation
S1090 Phosphorylation
Y1141 Phosphorylation

Research Backgrounds

Function:

Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).

May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.

Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

PTMs:

On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Basolateral cell membrane.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa. Isoform B and isoform A are expressed by NK cells (at protein level).

Subunit Structure:

Present as a mixture of monomers and dimers (Probable). The phosphorylated receptor binds a number of SH2 domain-containing proteins such as JAK2, STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits JAK/STAT signaling and MAPK cascade (By similarity).

Family&Domains:

The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

References

1). Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)D3 is mediated by inhibition of chaperone-mediated autophagy. Stem Cell Research & Therapy, 2021 (PubMed: 34717752) [IF=7.5]

2). OB-RGRP regulates the phosphorylation of JAK2 and STAT3 in primary rat adipocytes. Artificial Cells Nanomedicine and Biotechnology, 2019 (PubMed: 31841394) [IF=5.8]

Application: WB    Species: Rat    Sample:

Figure 3. The relative mRNA (A) and protein (B) levels of OB-RGRP in the cells transfected with OB-RGRP siRNA and expression vector which were determined by RT-PCR and western blot, respectively. si-OB-RGRP1: OB-RGRP-siRNA-1; si-OB-RGRP2: OB-RGRP-siRNA-2; si-OB-RGRP3: OB-RGRP-siRNA-3; si-OB-RGRP NC: OB-RGRP-siRNA negative control; Vector: empty expression vector; OB-RGRP: OB-RGRP expression vector. *p 

3). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods, 2020 [IF=5.6]

Application: WB    Species: Mouse    Sample: hypothalamus

Fig. 12. AGL regulates the leptin-mediated related signaling molecules. The expression of LEPR, AGPR, NPY, POMC and GAD65 in the hypothalamus assessed by western blotting. Data are presented as the means ± SD of more than 3 independent experiments. *p 

4). Leptin combined with withaferin A boost posthemorrhagic neurogenesis via activation of STAT3/SOCS3 pathway. Experimental neurology, 2024 (PubMed: 38714285) [IF=5.3]

5). Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring. Scientific Reports, 2023 (PubMed: 37355724) [IF=4.6]

Application: IHC    Species: Rat    Sample:

Figure 4 Quantitative analysis of the intensity of leptin IR in (A) villi and (B) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (C) Representative images of the leptin IR in sections of duodenum. Quantitative analysis of the intensity of leptin receptor IR in (D) villi and (E) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (F) Representative images of the ghrelin IR in sections of proximal jejunum. (C), (F): All scale bars represent 100 µm. (A), (B), (D), (E): Bar plots show lsmeans value and standard deviation (whiskers). A p-value range was attributed above plots when two groups exhibit significant differences: *p value 

6). Effects of the Water Extract of Fermented Rice Bran on Liver Damage and Intestinal Injury in Aged Rats with High-Fat Diet Feeding. Plants, 2022 (PubMed: 35270077) [IF=4.5]

Application: WB    Species: Rat    Sample:

Figure 5 Effects of the water extract of fermented rice bran (FRB) on hepatic lipid metabolism-related protein expressions in aged rats with high-fat (HF) diet feeding. Values are presented as the mean ± standard deviation (n = 6). * p < 0.05 vs. the normal control (NC) group; significance between two groups was determined using Student’s t-test. In the HF diet-fed groups, significant differences between groups were determined by a one-way ANOVA with Fisher‘s post hoc test. Western blot analysis of (A) NAD-dependent sirtuin-1 (SIRT1), (B) adenosine monophosphate-activated protein kinase-α (AMPKα), (C) phosphorylated (p)-AMPKα, (D) p-AMPKα/AMPKα, (E) leptin receptor, and (F) adiponectin receptor 2 (AdipoR2) protein expressions. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio of each internal control were calculated by setting the value of the mean of the NC group.

7). Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins. Animals, 2023 (PubMed: 37174575) [IF=3.0]

Application: IHC    Species: Rat    Sample:

Figure 2 Representative images and quantitative analysis of the intensity of ghrelin, ghrelin receptor, leptin, and leptin receptor immunoreaction (IR) in crypts and villi of the duodenum of female newborn rat offspring after maternal exposure to fumonisins (FBs) at the doses of 0, 60, or 90 mg/kg b.w. Bottom isotype row shows IHC IR control incubated without a primary antibody. All of the images were taken at the same magnification; scale bars represent 50 μm. Bar charts: intensity of immunoreaction (IR) to specific antibody was measured in crypts and villi using optical density (OD) measurements in the microscopic images. Results are presented as mean and SD. Statistically significant differences between groups are indicated by

8). Neuroprotective Effects of Leptin on the APP/PS1 Alzheimer’s Disease Mouse Model: Role of Microglial and Neuroinflammation. Degenerative Neurological and Neuromuscular Disease, 2023

Application: IHC    Species: Mouse    Sample: hippocampus

Figure 4 Leptin increased microglial cell number in the hippocampus of adult mice, but not aged mice. (A–D) Representative images of Iba-1+ cells, in the hippocampus sections of the mice. (E–G) Quantitative analysis of Iba-1+ stained cells.

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