Product: GLB1 Antibody
Catalog: DF6667
Description: Rabbit polyclonal antibody to GLB1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 76kDa; 76kD(Calculated).
Uniprot: P16278
RRID: AB_2838629

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
GLB1 Antibody detects endogenous levels of total GLB1.
RRID:
AB_2838629
Cite Format: Affinity Biosciences Cat# DF6667, RRID:AB_2838629.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acid beta galactosidase; Acid beta-galactosidase; Beta galactosidase 1; Beta galactosidase; Beta-galactosidase; BGAL_HUMAN; EBP; EBP, included; Elastin receptor 1 (67kD); Elastin receptor 1 67kDa; Elastin receptor 1; Elastin receptor 1, included; Elastin-binding protein, included; ELNR1; Galactosidase beta 1; GLB 1; GLB1; Lactase; MPS4B; S-GAL, included;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P16278 BGAL_HUMAN:

Detected in placenta (at protein level) (PubMed:8383699). Detected in fibroblasts and testis (PubMed:2511208).

Description:
This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MPGFLVRILPLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

PTMs - P16278 As Substrate

Site PTM Type Enzyme
T21 Phosphorylation
S37 Phosphorylation
S40 Phosphorylation
K43 Methylation
K43 Ubiquitination
Y57 Phosphorylation
S58 Phosphorylation
Y154 Phosphorylation
N247 N-Glycosylation
T286 Phosphorylation
Y294 Phosphorylation
S368 Phosphorylation
T369 Phosphorylation
K383 Ubiquitination
N464 N-Glycosylation
Y485 Phosphorylation
Y488 Phosphorylation
K493 Acetylation
N498 N-Glycosylation
N555 N-Glycosylation
K670 Ubiquitination

Research Backgrounds

Function:

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

Subcellular Location:

Lysosome.

Cytoplasm>Perinuclear region.
Note: Localized to the perinuclear area of the cytoplasm but not to lysosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in placenta (at protein level). Detected in fibroblasts and testis.

Subunit Structure:

Homodimer. May form higher multimers (Probable).

Family&Domains:

Belongs to the glycosyl hydrolase 35 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan degradation.

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - ganglio series.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Role of DNA damage response in cyclophosphamide-induced premature ovarian failure in mice. The journal of obstetrics and gynaecology research, 2024 (PubMed: 38936810) [IF=1.6]

Application: IHC    Species: Mouse    Sample:

FIGURE 4 Effects of cyclophosphamide (CTX) on Ki67, β-gal, p21, p53, γH2AX, and pATM protein expression. Immunohistochemistry was used to observe the cellular locations of these proteins.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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