Product: CXCL10 Antibody
Catalog: DF6417
Description: Rabbit polyclonal antibody to CXCL10
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 10kDa; 11kD(Calculated).
Uniprot: P02778
RRID: AB_2838380

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Horse(82%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
CXCL10 Antibody detects endogenous levels of total CXCL10.
RRID:
AB_2838380
Cite Format: Affinity Biosciences Cat# DF6417, RRID:AB_2838380.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;Interferon gamma induced factor MOB1, mouse, homolog of; Interferon gamma induced protein 10; 10 kDa interferon gamma induced protein; 10 kDa interferon gamma-induced protein; C X C motif chemokine 10; C7; Chemokine (C X C motif) ligand 10; Chemokine CXC motif ligand 10; Crg 2; CRG2; CXCL10; CXCL10(1-73); CXL10_HUMAN; Gamma IP10; Gamma-IP10; gIP 10; GIP10; IFI10; INP 10; INP10; Interferon activated gene 10; Interferon activated gene 10; Interferon gamma induced cell line; Interferon inducible cytokine IP 10; Interferon inducible cytokine IP10; IP 10; IP-10; Mob 1; MOB1; Protein 10 from interferon (gamma) induced cell line; SCYB10; Small inducible cytokine B10; Small inducible cytokine B10 precursor; Small inducible cytokine subfamily B (Cys X Cys) member 10; Small inducible cytokine subfamily B CXC member 10; Small inducible cytokine subfamily B, member 10; Small-inducible cytokine B10;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P02778 CXL10_HUMAN:

Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus (PubMed:11157474). Microglial cells produce CXCL10 in response to viral stimulation (PubMed:12663757).

Description:
This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Jul 2008]
Sequence:
MNQTAILICCLIFLTLSGIQGVPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
82
Rabbit
80
Bovine
64
Dog
64
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02778 As Substrate

Site PTM Type Enzyme
K67 Acetylation
K68 Acetylation
K69 Acetylation
K72 Acetylation
K80 Acetylation

Research Backgrounds

Function:

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).

PTMs:

Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation.

Subunit Structure:

Monomer, dimer, and tetramer. Interacts with CXCR3 (via N-terminus).

Family&Domains:

Belongs to the intercrine alpha (chemokine CxC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). Polystyrene microplastics induced male reproductive toxicity in mice. JOURNAL OF HAZARDOUS MATERIALS, 2021 (PubMed: 32659591) [IF=13.6]

2). Pterostilbene, A Bioactive Component of Blueberries, Alleviates Renal Interstitial Fibrosis by Inhibiting Macrophage-Myofibroblast Transition. The American journal of Chinese medicine, 2020 (PubMed: 33148003) [IF=5.7]

Application: WB    Species: Human    Sample: MMT cells

Figure 5. PTB mediated MMT through CXCL10. (A) Gene expression difference of volcano plot between Sham and UUO. (B) Gene expression difference of volcano plot between UUO and PTB treatment. (C) Venn plot suggests that 11 genes may play a role in the inhibition of RIF by PTB. (D) Heatmap analysis. (E) The verification of 11 genes by RT-PCR. (F) The protein level of CXCL10 in vivo. (G) The Luciferase assay showed the regulatory relationship between PTB and CXCL10. *P < 0:05, **P < 0:01, ***P < 0:001, and ****P < 0:0001.

3). DCUN1D1, a new molecule involved in depigmentation via upregulating CXCL10. Experimental dermatology, 2023 (PubMed: 36541112) [IF=3.6]

4). Evidence for the presence of Borrelia burgdorferi in invasive breast cancer tissues. European journal of microbiology & immunology, 2024 (PubMed: 38451280) [IF=2.2]

Application: IF/ICC    Species: Human    Sample: breast cancer tissues

Fig. 3. Representative images of CXCL8 and CXCL10 inflammatory markers on B. burgdorferi-positive and negative breast cancer and normal breast tissues. Panels: B and C show B. burgdorferi-positive breast cancer tissues stained Borrelia monoclonal antibody (green) and CXCL8 monoclonal antibody (red). Panels: F and G show B. burgdorferi-positive breast cancer tissues stained Borrelia monoclonal antibody (green) and CXCL10 monoclonal antibody (red). Panels: D and H show no staining on B. burgdorferi-negative breast cancer tissue samples for both inflammatory markers, CXCL8 and CXCL10 respectively. Panels: A and E show negative control; normal breast tissues are negative for B. burgdorferi and both inflammatory markers. White scale bar: 100 µm

5). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 3. Aquaporin 5 (AQP5) and Na-K-Cl cotransporter 1 (NKCC1) protein expression levels in sweat gland cells extracted from patients with primary palmar hyperhidrosis treated with blank control or transfected with overexpression negative control (OE NC) or integrin β6 (ITGB6) OE. Western blot images (A) and quantitative analyses (B) (ITGB6 OE compared to control, AQP5: Tukey’s honest significant difference (HSD), p = 0.055; NKCC1: Tukey’s HSD, p = 0.190). The data are presented as mean ± 95% confidence interval (95% CI)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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