Metadherin Antibody - #BF0508
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# BF0508, RRID:AB_2833740.
Fold/Unfold
3D3; 3D3/LYRIC; AEG 1; AEG-1; AEG1; Astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; LYRIC_HUMAN; Lysine rich CEACAM1 associated protein; Lysine rich CEACAM1 co isolated protein; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; Protein LYRIC;
Immunogens
Purified recombinant fragment of human Metadherin expressed in E. Coli.
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
- Q86UE4 LYRIC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAARSWQDELAQQAEEGSARLREMLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYGWAAACAGARKKRRSPPRKREEAAAVPAAAPDDLALLKNLRSEEQKKKNRKKLSEKPKPNGRTVEVAEGEAVRTPQSVTAKQPPEIDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVLTDSGSLDSTIPGIENTITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKTEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET
PTMs - Q86UE4 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S84 | Phosphorylation | Uniprot | |
K88 | Ubiquitination | Uniprot | |
K107 | Ubiquitination | Uniprot | |
S111 | Phosphorylation | Uniprot | |
K121 | Ubiquitination | Uniprot | |
K125 | Ubiquitination | Uniprot | |
K127 | Ubiquitination | Uniprot | |
T143 | Phosphorylation | Uniprot | |
S146 | Phosphorylation | Uniprot | |
T148 | Phosphorylation | Uniprot | |
K150 | Ubiquitination | Uniprot | |
K170 | Acetylation | Uniprot | |
K174 | Acetylation | Uniprot | |
K174 | Ubiquitination | Uniprot | |
S179 | Phosphorylation | Uniprot | |
S180 | Phosphorylation | Uniprot | |
K185 | Ubiquitination | Uniprot | |
K195 | Ubiquitination | Uniprot | |
S197 | Phosphorylation | Uniprot | |
S214 | Phosphorylation | Uniprot | |
S216 | Phosphorylation | Uniprot | |
S219 | Phosphorylation | Uniprot | |
K248 | Ubiquitination | Uniprot | |
S251 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
K261 | Methylation | Uniprot | |
K264 | Methylation | Uniprot | |
S267 | Phosphorylation | Uniprot | |
T268 | Phosphorylation | Uniprot | |
S272 | Phosphorylation | Uniprot | |
S273 | Phosphorylation | Uniprot | |
T280 | Phosphorylation | Uniprot | |
K289 | Ubiquitination | Uniprot | |
K292 | Ubiquitination | Uniprot | |
S294 | Phosphorylation | Uniprot | |
S295 | Phosphorylation | Uniprot | |
S298 | Phosphorylation | Uniprot | |
K303 | Ubiquitination | Uniprot | |
S306 | Phosphorylation | Uniprot | |
S308 | Phosphorylation | Uniprot | |
S311 | Phosphorylation | Uniprot | |
K314 | Acetylation | Uniprot | |
K314 | Methylation | Uniprot | |
K314 | Ubiquitination | Uniprot | |
S323 | Phosphorylation | Uniprot | |
K334 | Ubiquitination | Uniprot | |
R338 | Methylation | Uniprot | |
S339 | Phosphorylation | Uniprot | |
S341 | Phosphorylation | Uniprot | |
S344 | Phosphorylation | Uniprot | |
S347 | Phosphorylation | Uniprot | |
S357 | Phosphorylation | Uniprot | |
T361 | Phosphorylation | Uniprot | |
S362 | Phosphorylation | Uniprot | |
Y364 | Phosphorylation | Uniprot | |
S369 | Phosphorylation | Uniprot | |
S411 | Phosphorylation | Uniprot | |
S415 | Phosphorylation | Uniprot | |
S426 | Phosphorylation | Uniprot | |
T439 | Phosphorylation | Uniprot | |
K441 | Acetylation | Uniprot | |
S457 | Phosphorylation | Uniprot | |
T458 | Phosphorylation | Uniprot | |
T462 | Phosphorylation | Uniprot | |
S478 | Phosphorylation | Uniprot | |
K491 | Methylation | Uniprot | |
K491 | Ubiquitination | Uniprot | |
T492 | Phosphorylation | Uniprot | |
S494 | Phosphorylation | Uniprot | |
T495 | Phosphorylation | Uniprot | |
S496 | Phosphorylation | Uniprot | |
S506 | Phosphorylation | Uniprot | |
K510 | Ubiquitination | Uniprot | |
T511 | Phosphorylation | Uniprot | |
S521 | Phosphorylation | Uniprot | |
S527 | Phosphorylation | Uniprot | |
S529 | Phosphorylation | Uniprot | |
S532 | Phosphorylation | Uniprot | |
S534 | Phosphorylation | Uniprot | |
S546 | Phosphorylation | Uniprot | |
S548 | Phosphorylation | Uniprot | |
T550 | Phosphorylation | Uniprot | |
S558 | Phosphorylation | Uniprot | |
T560 | Phosphorylation | Uniprot | |
S562 | Phosphorylation | Uniprot | |
T564 | Phosphorylation | Uniprot | |
S565 | Phosphorylation | Uniprot | |
S568 | Phosphorylation | Uniprot | |
K570 | Ubiquitination | Uniprot | |
T582 | Phosphorylation | Uniprot |
Research Backgrounds
Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein. Cell junction>Tight junction. Nucleus>Nucleolus. Cytoplasm>Perinuclear region.
Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
Interacts with BCCIP, CREBBP/CBP and RELA/p65.
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