Product: PAI 2 Antibody
Catalog: AF5174
Description: Rabbit polyclonal antibody to PAI 2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 46 kDa; 47kD(Calculated).
Uniprot: P05120
RRID: AB_2837660

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(85%), Rabbit(85%), Dog(85%)
Clonality:
Polyclonal
Specificity:
PAI 2 Antibody detects endogenous levels of total PAI 2.
RRID:
AB_2837660
Cite Format: Affinity Biosciences Cat# AF5174, RRID:AB_2837660.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HsT1201; Monocyte Arg serpin; Monocyte Arg-serpin; Monocyte Arginine-serpin; Monocyte-derived plasminogen activator inhibitor; PAI; PAI-2; PAI2; PAI2_HUMAN; Placental plasminogen activator inhibitor; PLANH2; Plasminogen activator inhibitor 2; plasminogen activator inhibitor, type 2; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Serpinb2; Urokinase inhibitor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-2.
Sequence:
MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
85
Dog
85
Rabbit
85
Pig
67
Bovine
62
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05120 As Substrate

Site PTM Type Enzyme
T69 Phosphorylation
T72 Phosphorylation
S136 Phosphorylation
S171 Phosphorylation
T175 Phosphorylation
T177 Phosphorylation
S296 Phosphorylation

Research Backgrounds

Function:

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

PTMs:

The signal sequence is not cleaved.

Subcellular Location:

Cytoplasm. Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PSMB1.

Family&Domains:

Belongs to the serpin family. Ov-serpin subfamily.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Chelerythrine chloride inhibits the progression of colorectal cancer by targeting cancer-associated fibroblasts through intervention with WNT10B/β-catenin and TGFβ2/Smad2/3 axis. Phytotherapy research : PTR, 2023 (PubMed: 37402476) [IF=7.2]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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