GIPR Antibody - #DF5176

FIGURE 2 Gastric inhibitory peptide (GlP) and GlP receptor (GlPR) is upregulated in response to sciatic nerve transection injury.Representative realtime reverse transcription-polymerase chain reaction data showing the relative mRNA levels of GlP (A, B) and GIPR (CD) in the proximal (A, C) and distal (B, D) nerve fragments, respectively, at each time point after injury indicated (dpi, days post injury; n=4Both GlP and GlPR were upregulated in the proximal nerve fragment, while the results were slightly different in the distal nerve fragmentsData were presented as means +standard error of mean (SEM). (E-H) Although GlPR mRNA levels were little changed in distal nerveresidues, western blot results showed that GlPR protein expression was upregulated in both proximal and distal nerve fragments after injuryp-actin levels are shown as a loading control. (l) Representative immunostaining images of normal (a) and injured sciatic nerve segments (c.Green color indicates $100p,red color indicates GlPR, and blue color indicates nucleus. Scale bars=300ym. Boxed areas are demonstratedat a higher magnification on the right (b,d, and e). Scale bars=2um. A one-way ANOVA was used to assess the outcome measures fromtime (A-D, G, H). Data were presented as means+SEM,n=4 animals/group (A, B), 5 animals/group (c, D) or 3 animals/group (G, H). *"pvalue