Product: RHOD Antibody
Catalog: DF4439
Description: Rabbit polyclonal antibody to RHOD
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 25 KD; 23kD(Calculated).
Uniprot: O00212
RRID: AB_2836794

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Bovine(83%), Horse(92%), Sheep(92%), Dog(83%)
Clonality:
Polyclonal
Specificity:
RHOD Antibody detects endogenous levels of total RHOD.
RRID:
AB_2836794
Cite Format: Affinity Biosciences Cat# DF4439, RRID:AB_2836794.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHD; Ras homolog D; Ras homolog gene family member A; Ras homolog gene family member D; Rho; RHO D; Rho related GTP binding protein RhoD; Rho related protein HP1; Rho-related GTP-binding protein RhoD; Rho-related protein HP1; RHOD; RHOD_HUMAN; RhoHP1; RHOM;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O00212 RHOD_HUMAN:

Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.

Sequence:
MTAAQAAGEEAPPGVRSVKVVLVGDGGCGKTSLLMVFADGAFPESYTPTVFERYMVNLQVKGKPVHLHIWDTAGQDDYDRLRPLFYPDASVLLLCFDVTSPNSFDNIFNRWYPEVNHFCKKVPIIVVGCKTDLCKDKSLVNKLRRNGLEPVTYHRGQEMARSVGAVAYLECSARLHDNVHAVFQEAAEVALSSRGRNFWRRITQGFCVVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Sheep
92
Bovine
83
Dog
83
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00212 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
K19 Ubiquitination
K63 Ubiquitination
K121 Ubiquitination
K137 Ubiquitination
S138 Phosphorylation

Research Backgrounds

Function:

Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome.
Note: Colocalizes with RAB5 to early endosomes (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.

Subunit Structure:

Interacts (in GTP-bound form) with DIAPH2 isoform 3, DAPK3, FILIP1 and WHAMM. Interacts with PAK5. Interacts (independent of GTP-loaded status) with ANKFY1.

Family&Domains:

Belongs to the small GTPase superfamily. Rho family.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Anoikis regulator GLI2 promotes NC cell immunity escape by TGF-β-mediated non-classic hedgehog signaling in colorectal cancer: based on artificial intelligence and big data analysis. Aging, 2023 (PubMed: 38159250) [IF=5.2]

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