Product: OAT Antibody
Catalog: DF4270
Description: Rabbit polyclonal antibody to OAT
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 48 KD; 49kD(Calculated).
Uniprot: P04181
RRID: AB_2836621

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Chicken(91%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
OAT Antibody detects endogenous levels of total OAT.
RRID:
AB_2836621
Cite Format: Affinity Biosciences Cat# DF4270, RRID:AB_2836621.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GACR; Gyrate atrophy; HOGA; OAT; OATASE; OKT; Ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase mitochondrial; ornithine aminotransferase precursor; Ornithine aminotransferase, mitochondrial precursor; Ornithine delta aminotransferase; Ornithine oxo acid aminotransferase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MFSKLAHLQRFAVLSRGVHSSVASATSVATKKTVQGPPTSDDIFEREYKYGAHNYHPLPVALERGKGIYLWDVEGRKYFDFLSSYSAVNQGHCHPKIVNALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
91
Pig
82
Zebrafish
82
Rabbit
80
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04181 As Substrate

Site PTM Type Enzyme
T30 Phosphorylation
K32 Ubiquitination
T39 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
K66 Ubiquitination
Y69 Phosphorylation
Y85 Phosphorylation
K96 Acetylation
K102 Ubiquitination
Y123 Phosphorylation
Y126 Phosphorylation
Y133 Phosphorylation
K151 Ubiquitination
T272 Phosphorylation
Y280 Phosphorylation
C306 S-Nitrosylation
C330 S-Nitrosylation
K362 Ubiquitination
S365 Phosphorylation
K374 Ubiquitination
K386 Ubiquitination
K392 Ubiquitination
K405 Ubiquitination
R426 Methylation

Research Backgrounds

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homohexamer.

Family&Domains:

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

2). Inactivation of PDH can Reduce Anaplastic Thyroid Cancer Cells' Sensitivity to Artemisinin. Anti-Cancer Agents in Medicinal Chemistry, 2022 (PubMed: 34515013) [IF=2.8]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.