AFfirm™ Cyclin E1 Mouse Monoclonal Antibody - #BF8955
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
CCNE; Ccne1; CCNE1_HUMAN; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E; G1/S-specific cyclin-E1;
PTMs - P24864 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S59 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| T77 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S90 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S391 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | P49841 (GSK3B) , P24941 (CDK2) , P49840 (GSK3A) | Uniprot |
| S399 | Phosphorylation | P24941 (CDK2) | Uniprot |
Research Backgrounds
Essential for the control of the cell cycle at the G1/S (start) transition.
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
Nucleus.
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Interacts with INCA1.
Belongs to the cyclin family. Cyclin E subfamily.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
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