Product: PDCD4 Mouse Monoclonal Antibody
Catalog: BF8938
Description: Mouse monoclonal antibody to PDCD4
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 56kDa; 52kD(Calculated).
Uniprot: Q53EL6

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8938]
Specificity:
PDCD4 Mouse Monoclonal Antibody detects endogenous levels of total PDCD4.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein;

PTMs - Q53EL6 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S20 Phosphorylation
S22 Phosphorylation
S25 Phosphorylation
S46 Phosphorylation
K58 Ubiquitination
K60 Ubiquitination
S67 Phosphorylation P31749 (AKT1) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)
S68 Phosphorylation
S71 Phosphorylation
R73 Methylation
S76 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
S87 Phosphorylation
T90 Phosphorylation
T93 Phosphorylation
S94 Phosphorylation
K96 Ubiquitination
K121 Ubiquitination
T126 Phosphorylation
Y131 Phosphorylation
Y143 Phosphorylation
Y152 Phosphorylation
K166 Ubiquitination
K212 Ubiquitination
K238 Ubiquitination
K242 Ubiquitination
K245 Ubiquitination
T254 Phosphorylation
R267 Methylation
K283 Ubiquitination
K297 Ubiquitination
T299 Phosphorylation
S303 Phosphorylation
K306 Ubiquitination
K311 Ubiquitination
S313 Phosphorylation
S317 Phosphorylation
S323 Phosphorylation
S345 Phosphorylation
K392 Ubiquitination
K402 Ubiquitination
Y405 Phosphorylation
S440 Phosphorylation
S457 Phosphorylation P31749 (AKT1)
K465 Sumoylation
S468 Phosphorylation

Research Backgrounds

Function:

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

PTMs:

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Subunit Structure:

Interacts (via MI domains) with EIF4A2 (By similarity). Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11.

Family&Domains:

Binds EIF4A1 via both MI domains.

Belongs to the PDCD4 family.

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.