Product: FRS2 Mouse Monoclonal Antibody
Catalog: BF8954
Description: Mouse monoclonal antibody to FRS2
Application: WB
Reactivity: Human
Prediction: Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 65~85kDa; 57kD(Calculated).
Uniprot: Q8WU20

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8954]
Specificity:
FRS2 Mouse Monoclonal Antibody detects endogenous levels of total FRS2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGFR signaling adaptor SNT; FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS 2; FRS2; FRS2_HUMAN; FRS2A; FRS2alpha; SNT 1; SNT; SNT-1; SNT1; Suc 1 Associated Neurotrophic Factor Target; Suc1 associated neurotrophic factor target 1; Suc1-associated neurotrophic factor target 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8WU20 FRS2_HUMAN:

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Description:
FRS2 is an adaptor protein involved in fibroblast growth factor receptor (FGFR) signaling. Plays an important role in linking FGFR and nerve growth factor receptors with Ras/MAPK signaling pathways.
Sequence:
MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPSVNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYENLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKIEYSRRRDCTPTVFNFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSNLQKALPRDDGTSRKTRHNSTDLPM

PTMs - Q8WU20 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
T49 Phosphorylation
Y59 Phosphorylation
S73 Phosphorylation
S76 Phosphorylation
S109 Phosphorylation
T125 Phosphorylation
T132 Phosphorylation
T135 Phosphorylation
T137 Phosphorylation
T138 Phosphorylation
Y150 Phosphorylation
Y153 Phosphorylation
S155 Phosphorylation
S169 Phosphorylation
S177 Phosphorylation
T183 Phosphorylation
Y196 Phosphorylation
T200 Phosphorylation
S211 Phosphorylation
S221 Phosphorylation
S226 Phosphorylation
T227 Phosphorylation
K267 Ubiquitination
Y290 Phosphorylation
S292 Phosphorylation
S300 Phosphorylation
Y306 Phosphorylation
S313 Phosphorylation
S316 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
T328 Phosphorylation
S329 Phosphorylation
T344 Phosphorylation
Y349 Phosphorylation
S354 Phosphorylation
S365 Phosphorylation
T376 Phosphorylation
S378 Phosphorylation
Y392 Phosphorylation
T395 Phosphorylation
S403 Phosphorylation
S428 Phosphorylation
Y436 Phosphorylation
S445 Phosphorylation
S447 Phosphorylation
T452 Phosphorylation
T455 Phosphorylation
T457 Phosphorylation
T458 Phosphorylation
T463 Phosphorylation
T465 Phosphorylation
T468 Phosphorylation
Y471 Phosphorylation
T479 Phosphorylation
S483 Phosphorylation
T499 Phosphorylation
S503 Phosphorylation
T504 Phosphorylation

Research Backgrounds

Function:

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

PTMs:

Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by NGF or FGF2. Phosphorylated on tyrosine residues by activated ALK and FGFR1. Phosphorylated on tyrosine residues upon activation of FGFR2 and FGFR3. Phosphorylated on threonine residues by MAP kinases; this inhibits tyrosine phosphorylation, and thereby down-regulates FRS2-mediated activation of MAP kinases.

Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination (By similarity).

Subcellular Location:

Endomembrane system.
Note: Cytoplasmic, membrane-bound.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

Subunit Structure:

Part of a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Part of a complex containing GRB2 and CBL. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds RET (By similarity). Binds ALK, FGFR1, CKS2, MAPK1/ERK2, MAPK3/ERK1 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated upon ligand-binding).

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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