Product: MAT1A Mouse Monoclonal Antibody
Catalog: BF8854
Description: Mouse monoclonal antibody to MAT1A
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 44-55 kDa; 44kD(Calculated).
Uniprot: Q00266

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8854]
Specificity:
MAT1A Mouse Monoclonal Antibody detects endogenous levels of total MAT1A.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AdoMet; AdoMet synthetase 1; AI046368; Ams; MAT; MAT I/III; MATA1; Methionine adenosyltransferase 1; Methionine adenosyltransferase I, alpha; Methionine adenosyltransferase I/III; MGC108563; S adenosylmethionine synthetase isoform type 1; SADE; SAMS; SAMS1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q00266 METK1_HUMAN:

Expressed in liver.

Sequence:
MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

PTMs - Q00266 As Substrate

Site PTM Type Enzyme
S73 Phosphorylation
K223 Ubiquitination
R264 Methylation
K285 Ubiquitination
K289 Ubiquitination
S293 Phosphorylation
K303 Ubiquitination
T341 Phosphorylation
K351 Ubiquitination

Research Backgrounds

Function:

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

PTMs:

S-nitrosylation of Cys-120 inactivates the enzyme.

An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.

Tissue Specificity:

Expressed in liver.

Subunit Structure:

Homotetramer (MAT-I); dimer of dimers. Homodimer (MAT-III) (By similarity).

Family&Domains:

Belongs to the AdoMet synthase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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