Product: NASP Mouse Monoclonal Antibody
Catalog: BF8851
Description: Mouse monoclonal antibody to NASP
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 85,150kD; 85kD(Calculated).
Uniprot: P49321

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8851]
Specificity:
NASP Mouse Monoclonal Antibody detects endogenous levels of total NASP.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P49321 NASP_HUMAN:

Isoform 1 is testis- and sperm-specific.

Sequence:
MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTEAAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC

PTMs - P49321 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K20 Acetylation
S28 Phosphorylation
S30 Phosphorylation
K33 Acetylation
K33 Ubiquitination
S36 Phosphorylation
S41 Phosphorylation
K45 Ubiquitination
K52 Ubiquitination
S71 Phosphorylation
K75 Ubiquitination
K76 Ubiquitination
K93 Ubiquitination
S94 Phosphorylation
T123 Phosphorylation
S127 Phosphorylation
R140 Methylation
Y148 Phosphorylation
K154 Acetylation
T170 Phosphorylation
S176 Phosphorylation
K180 Acetylation
S189 Phosphorylation
S191 Phosphorylation
T202 Phosphorylation
S210 Phosphorylation
T228 Phosphorylation
K231 Ubiquitination
S244 Phosphorylation
S246 Phosphorylation
T248 Phosphorylation
C254 S-Nitrosylation
S269 Phosphorylation
T291 Phosphorylation
S299 Phosphorylation
S321 Phosphorylation
S344 Phosphorylation
S384 Phosphorylation
T390 Phosphorylation
T396 Phosphorylation
S397 Phosphorylation
T402 Phosphorylation
K405 Ubiquitination
S408 Phosphorylation
K413 Ubiquitination
K417 Acetylation
K417 Ubiquitination
S421 Phosphorylation
T425 Phosphorylation
K426 Ubiquitination
S428 Phosphorylation
S432 Phosphorylation
K442 Ubiquitination
T448 Phosphorylation
K450 Acetylation
S451 Phosphorylation
K455 Acetylation
T464 Phosphorylation
T477 Phosphorylation
S480 Phosphorylation
T490 Phosphorylation
S497 Phosphorylation
S503 Phosphorylation
T534 Phosphorylation
K535 Ubiquitination
Y540 Phosphorylation
S567 Phosphorylation
Y575 Phosphorylation
S598 Phosphorylation
R617 Methylation
K626 Ubiquitination
K636 Sumoylation
K643 Acetylation
K643 Ubiquitination
K652 Ubiquitination
K657 Ubiquitination
S659 Phosphorylation
S662 Phosphorylation
K671 Ubiquitination
T683 Phosphorylation
S689 Phosphorylation
S690 Phosphorylation
S696 Phosphorylation
K698 Acetylation
K698 Ubiquitination
T700 Phosphorylation
S704 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
S713 Phosphorylation
K721 Methylation
S726 Phosphorylation
K733 Acetylation
K734 Acetylation
K736 Acetylation
K736 Sumoylation
S745 Phosphorylation
S751 Phosphorylation
S756 Phosphorylation
C788 S-Nitrosylation

Research Backgrounds

Function:

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is testis- and sperm-specific.

Subunit Structure:

Binds to linker H1 histones but not to core histones (By similarity). Interacts with histones H2A, H2B, H3 and H4. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).

Family&Domains:

Belongs to the NASP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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