Product: DIAPH1 Mouse Monoclonal Antibody
Catalog: BF8847
Description: Mouse monoclonal antibody to DIAPH1
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Horse, Sheep, Rabbit, Dog, Monkey
Mol.Wt.: 140-150, 70 kDa; 141kD(Calculated).
Uniprot: O60610

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 100ul $280 In stock
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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8847]
Specificity:
DIAPH1 Mouse Monoclonal Antibody detects endogenous levels of total DIAPH1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DIAPH1; deafness, autosomal dominant 1; DFNA1; DIA1; DIAP1; DIAP1_HUMAN; DIAPH1; Diaphanous homolog 1 (Drosophila); diaphanous homolog 1; Diaphanous related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1; LFHL1; low frequency hearing loss 1; p140DIA; Protein diaphanous homolog 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O60610 DIAP1_HUMAN:

Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets (PubMed:26912466).

Sequence:
MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS

PTMs - O60610 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation
R12 Methylation
T14 Phosphorylation
S22 Phosphorylation
S28 Phosphorylation
K35 Acetylation
K35 Methylation
K35 Ubiquitination
S36 Phosphorylation
K37 Acetylation
K38 Acetylation
T40 Phosphorylation
K42 Methylation
S54 Phosphorylation
S71 Phosphorylation
Y75 Phosphorylation
Y128 Phosphorylation
Y130 Phosphorylation
T131 Phosphorylation
S132 Phosphorylation
K133 Ubiquitination
S154 Phosphorylation
K196 Ubiquitination
K202 Ubiquitination
Y209 Phosphorylation
K214 Ubiquitination
K222 Ubiquitination
K303 Ubiquitination
K311 Ubiquitination
S373 Phosphorylation
Y374 Phosphorylation
K377 Ubiquitination
Y424 Phosphorylation
K453 Ubiquitination
K473 Acetylation
K476 Acetylation
K476 Ubiquitination
K480 Acetylation
K486 Ubiquitination
K487 Ubiquitination
K503 Ubiquitination
S507 Phosphorylation
K520 Acetylation
K520 Ubiquitination
K527 Ubiquitination
S542 Phosphorylation
K550 Ubiquitination
T768 Phosphorylation P06493 (CDK1)
K771 Ubiquitination
Y773 Phosphorylation
K774 Ubiquitination
K786 Ubiquitination
K800 Ubiquitination
K802 Ubiquitination
K814 Acetylation
K824 Acetylation
K824 Ubiquitination
K830 Acetylation
K855 Ubiquitination
S866 Phosphorylation
Y871 Phosphorylation
K1046 Ubiquitination
S1051 Phosphorylation
K1057 Acetylation
K1057 Ubiquitination
T1091 Phosphorylation
K1103 Acetylation
K1103 Ubiquitination
Y1121 Phosphorylation
K1224 Ubiquitination
C1227 S-Nitrosylation
T1238 Phosphorylation
K1239 Ubiquitination
K1249 Ubiquitination
S1251 Phosphorylation
K1252 Ubiquitination
S1254 Phosphorylation
T1256 Phosphorylation
K1265 Ubiquitination
S1272 Phosphorylation

Research Backgrounds

Function:

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Plays a role in brain development. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity).

PTMs:

Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.

Subcellular Location:

Cell membrane. Cell projection>Ruffle membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm. Nucleus.
Note: Membrane ruffles, especially at the tip of ruffles, of motile cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets.

Subunit Structure:

Homodimer (By similarity). Interacts with the GTP-bound form of RHOA. Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC (By similarity). Interacts with SCAI (By similarity). Interacts with DCAF7, via FH2 domain (By similarity). Interacts with NCDN (By similarity). Interacts with OSBPL10, OSBPL2, VIM, TUBB and DYN1.

Family&Domains:

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain (By similarity). This autoinhibition is released upon competitive binding of an activated GTPase (By similarity). The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).

Belongs to the formin homology family. Diaphanous subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

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