Product: ACTN3 Mouse Monoclonal Antibody
Catalog: BF8653
Description: Mouse monoclonal antibody to ACTN3
Application: WB
Reactivity: Human
Prediction: Mouse, Rat
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: Q08043

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8653]
Specificity:
ACTN3 Mouse Monoclonal Antibody detects endogenous levels of total ACTN3.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actinin alpha 3; actinin, alpha; ACTN 3; ACTN3; ACTN3_HUMAN; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 3; Alpha-Actinin 3; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; F actin cross linking protein; F-actin cross-linking protein; MGC117002; MGC117005; OTTHUMP00000219049; OTTHUMP00000219054;

Immunogens

Immunogen:

A synthesized peptide derived from human ACTN3, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
Q08043 ACTN3_HUMAN:

Expressed only in a subset of type 2 skeletal muscle fibers.

Sequence:
MMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRIQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEELARQQVNERLRRQFAAQANAIGPWIQAKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKQNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTTEQVVASFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGALDYVAFSSALYGESDL

PTMs - Q08043 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K45 Ubiquitination
K49 Ubiquitination
K61 Ubiquitination
T64 Phosphorylation
R90 Methylation
R93 Methylation
S116 Phosphorylation
K117 Ubiquitination
T137 Phosphorylation
S154 Phosphorylation
T158 Phosphorylation
S159 Phosphorylation
K209 Sumoylation
K212 Sumoylation
Y229 Phosphorylation
K250 Acetylation
S257 Phosphorylation
Y260 Phosphorylation
R275 Methylation
K278 Acetylation
S298 Phosphorylation
S319 Phosphorylation
S321 Phosphorylation
Y333 Phosphorylation
T354 Phosphorylation
T357 Phosphorylation
S376 Phosphorylation
S418 Phosphorylation
Y436 Phosphorylation
S438 Phosphorylation
S456 Phosphorylation
K506 Acetylation
S737 Phosphorylation
Y851 Phosphorylation
Y876 Phosphorylation

Research Backgrounds

Function:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Tissue Specificity:

Expressed only in a subset of type 2 skeletal muscle fibers.

Subunit Structure:

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.

Family&Domains:

Belongs to the alpha-actinin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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