Product: CHST5 Mouse Monoclonal Antibody
Catalog: BF8643
Description: Mouse monoclonal antibody to CHST5
Application: WB
Reactivity: Human
Prediction: Human
Mol.Wt.: 46~55 kDa; 46kD(Calculated).
Uniprot: Q9GZS9

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Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8643]
Specificity:
CHST5 Mouse Monoclonal Antibody detects endogenous levels of total CHST5.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carbohydrate (N acetylglucosamine 6 O) sulfotransferase 5; Carbohydrate sulfotransferase 5; Chst5; CHST5_HUMAN; FLJ22167; Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 4 alpha; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha; GlcNAc6ST 3; GlcNAc6ST-3; Gn6st-3; GST4 alpha; GST4-alpha; hIGn6ST; I GlcNAc 6 ST; I GlcNAc6ST; I-GlcNAc6ST; IGlcNAc6ST; Intestinal GlcNAc 6 sulfotransferase; Intestinal GlcNAc-6-sulfotransferase; Intestinal GlcNAcv6 sulfotransferase; Intestinal N acetylglucosamine 6 O sulfotransferase; Intestinal N-acetylglucosamine-6-O-sulfotransferase; MGC74625; N acetylglucosamine 6 O sulfotransferase 3; N acetylglucosamine 6 O sulfotransferase; N-acetylglucosamine 6-O-sulfotransferase 3; OTTHUMP00000174950;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9GZS9 CHST5_HUMAN:

Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.

Sequence:
MGMRARVPKVAHSTRRPPAARMWLPRFSSKTVTVLLLAQTTCLLLFIISRPGPSSPAGGEDRVHVLVLSSWRSGSSFLGQLFSQHPDVFYLMEPAWHVWTTLSQGSAATLHMAVRDLMRSIFLCDMDVFDAYMPQSRNLSAFFNWATSRALCSPPACSAFPRGTISKQDVCKTLCTRQPFSLAREACRSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREAAGPILARDNGIVLGTNGKWVEADPHLRLIREVCRSHVRIAEAATLKPPPFLRGRYRLVRFEDLAREPLAEIRALYAFTGLTLTPQLEAWIHNITHGSGIGKPIEAFHTSSRNARNVSQAWRHALPFTKILRVQEVCAGALQLLGYRPVYSADQQRDLTLDLVLPRGPDHFSWASPD

PTMs - Q9GZS9 As Substrate

Site PTM Type Enzyme
S181 Phosphorylation
S189 Phosphorylation
Y190 Phosphorylation
S191 Phosphorylation

Research Backgrounds

Function:

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.
Note: Golgi membrane, early secretory pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.

Family&Domains:

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Restrictive clause

 

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