Product: ATP5A1 Mouse Monoclonal Antibody
Catalog: BF8068
Description: Mouse monoclonal antibody to ATP5A1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Chicken, Xenopus
Mol.Wt.: 60 KD; 60kD(Calculated).
Uniprot: P25705

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Mouse
Application:
WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8068]
Specificity:
ATP5A1 Antibody detects endogenous levels of total ATP5A1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP synthase alpha chain, mitochondrial; ATP synthase subunit alpha; ATP synthase subunit alpha mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1 ATPase) alpha subunit; ATP5A; Atp5a1; ATP5AL2; ATPA_HUMAN; ATPM; Epididymis secretory sperm binding protein Li 123m; hATP1; HEL-S-123m; MC5DN4; mitochondrial; Mitochondrial ATP synthetase; Mitochondrial ATP synthetase oligomycin resistant; Modifier of Min 2 mouse homolog; Modifier of Min 2, mouse, homolog of; MOM2; OMR; ORM; OTTHUMP00000163475;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P25705 ATPA_HUMAN:

Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Sequence:
MLSVRVAAAVVRALPRRAGLVSRNALGSSFIAARNFHASNTHLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA

PTMs - P25705 As Substrate

Site PTM Type Enzyme
K45 Ubiquitination
T48 Phosphorylation
S53 Phosphorylation
T64 Phosphorylation
S65 Phosphorylation
S76 Phosphorylation
R88 Methylation
S99 Phosphorylation
S100 Phosphorylation
K123 Acetylation
K126 Ubiquitination
K132 Acetylation
T134 Phosphorylation
K161 Acetylation
K161 Ubiquitination
S166 Phosphorylation
K167 Ubiquitination
K175 Ubiquitination
S184 Phosphorylation
K194 Ubiquitination
S198 Phosphorylation
R204 Methylation
R207 Methylation
R214 Methylation
K218 Ubiquitination
S220 Phosphorylation
T225 Phosphorylation
K230 Acetylation
K230 Ubiquitination
K239 Acetylation
K240 Acetylation
K241 Acetylation
Y243 Phosphorylation
C244 S-Nitrosylation
Y246 Phosphorylation
S254 Phosphorylation
T255 Phosphorylation
K261 Acetylation
K261 Ubiquitination
T264 Phosphorylation
K270 Acetylation
Y299 Phosphorylation
K305 Acetylation
K305 Ubiquitination
Y311 Phosphorylation
K316 Ubiquitination
Y337 Phosphorylation
Y343 Phosphorylation
S419 Phosphorylation
K427 Acetylation
K427 Ubiquitination
T432 Phosphorylation
K434 Acetylation
K434 Ubiquitination
Y440 Phosphorylation
S451 Phosphorylation
S462 Phosphorylation
S477 Phosphorylation
K498 Acetylation
K498 Ubiquitination
S502 Phosphorylation
K506 Acetylation
K506 Ubiquitination
K531 Acetylation
K531 Ubiquitination
K539 Acetylation
K539 Ubiquitination
K541 Ubiquitination

Research Backgrounds

Function:

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity). Binds the bacterial siderophore enterobactin and can promote mitochondrial accumulation of enterobactin-derived iron ions.

PTMs:

The N-terminus is blocked.

Acetylated on lysine residues. BLOC1S1 is required for acetylation.

Subcellular Location:

Mitochondrion. Mitochondrion inner membrane>Peripheral membrane protein>Matrix side. Cell membrane>Peripheral membrane protein>Extracellular side.
Note: Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.

Subunit Structure:

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c (By similarity). Interacts with ATPAF2. Interacts with HRG; the interaction occurs on the surface of T-cells and alters the cell morphology when associated with concanavalin (in vitro). Interacts with PLG (angiostatin peptide); the interaction inhibits most of the angiogenic properties of angiostatin. Component of an ATP synthase complex composed of ATP5PB, ATP5MC1, ATP5F1E, ATP5PD, ATP5ME, ATP5PF, ATP5MF, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5PO, ATP5MG, ATP5MD and ATP5MPL (By similarity). Interacts with BLOC1S1. Interacts with BCL2L1 isoform BCL-X(L); the interaction mediates the association of BCL2L1 isoform BCL-X(L) with the mitochondrial membrane F(1)F(0) ATP synthase and enhances neurons metabolic efficiency (By similarity). Interacts with CLN5 and PPT1 (By similarity).

Family&Domains:

Belongs to the ATPase alpha/beta chains family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Patchouli alcohol alleviates metabolic dysfunction-associated steatohepatitis via inhibiting mitochondria-associated endoplasmic reticulum membrane disruption-induced hepatic steatosis and inflammation in rats. International immunopharmacology, 2024 (PubMed: 38971107) [IF=5.6]

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