AP1G2 Antibody | Affinity Biosciences

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DF15085 at 1/100 staining rat muscle tissue by IHC-P.

Product:	AP1G2 Antibody
Catalog:	DF15085
Description:	Rabbit polyclonal antibody to AP1G2
Application:	IHC
Reactivity:	Human, Mouse
Mol.Wt.:	87kD(Calculated).
Uniprot:	O75843

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

AP1G2 Antibody detects endogenous levels of AP1G2.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AP-1 complex subunit gamma-like 2; Ap1g2; AP1G2_HUMAN; G2ad; Gamma2-adaptin;

Immunogens

Immunogen:

A synthesized peptide derived from human AP1G2.

Uniprot:

O75843(AP1G2_HUMAN) >>Visit HPA database.

Gene(ID):

AP1G2(8906)

Expression:

O75843 AP1G2_HUMAN:

Expressed in all but one (skeletal muscle) tissues examined.

Sequence:

O75843 AP1G2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVVPSLKLQDLIEEIRGAKTQAQEREVIQKECAHIRASFRDGDPVHRHRQLAKLLYVHMLGYPAHFGQMECLKLIASSRFTDKRVGYLGAMLLLDERHDAHLLITNSIKNDLSQGIQPVQGLALCTLSTMGSAEMCRDLAPEVEKLLLQPSPYVRKKAILTAVHMIRKVPELSSVFLPPCAQLLHERHHGILLGTITLITELCERSPAALRHFRKVVPQLVHILRTLVTMGYSTEHSISGVSDPFLQVQILRLLRILGRNHEESSETMNDLLAQVATNTDTSRNAGNAVLFETVLTIMDIRSAAGLRVLAVNILGRFLLNSDRNIRYVALTSLLRLVQSDHSAVQRHRPTVVECLRETDASLSRRALELSLALVNSSNVRAMMQELQAFLESCPPDLRADCASGILLAAERFAPTKRWHIDTILHVLTTAGTHVRDDAVANLTQLIGGAQELHAYSVRRLYNALAEDISQQPLVQVAAWCIGEYGDLLLAGNCEEIEPLQVDEEEVLALLEKVLQSHMSLPATRGYALTALMKLSTRLCGDNNRIRQVVSIYGSCLDVELQQRAVEYDTLFRKYDHMRAAILEKMPLVERDGPQADEEAKESKEAAQLSEAAPVPTEPQASQLLDLLDLLDGASGDVQHPPHLDPSPGGALVHLLDLPCVPPPPAPIPDLKVFEREGVQLNLSFIRPPENPALLLITITATNFSEGDVTHFICQAAVPKSLQLQLQAPSGNTVPARGGLPITQLFRILNPNKAPLRLKLRLTYDHFHQSVQEIFEVNNLPVESWQ

Research Backgrounds

O75843_AP1G2_HUMAN

Function:

May function in protein sorting in late endosomes or multivesucular bodies (MVBs).

(Microbial infection) Involved in MVB-assisted maturation of hepatitis B virus (HBV).

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein.
Note: Mainly localized to perinuclear vesicular structures (PubMed:9733768). Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments (PubMed:16867982). Colocalizes with HBV major surface antigen L to cis-Golgi-like structures (PubMed:11333915).

Tissue Specificity:

Expressed in all but one (skeletal muscle) tissues examined.

Subunit Structure:

Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Binds ubiquitin.

(Microbial infection) Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner.

Family&Domains:

Belongs to the adaptor complexes large subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

Restrictive clause

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AP1G2 Antibody - #DF15085