Histone H1.1 Antibody - #DF13743
Product: | Histone H1.1 Antibody |
Catalog: | DF13743 |
Description: | Rabbit polyclonal antibody to Histone H1.1 |
Application: | WB IF/ICC |
Reactivity: | Human |
Prediction: | Horse |
Mol.Wt.: | 21kDa; 22kD(Calculated). |
Uniprot: | Q02539 |
RRID: | AB_2846762 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13743, RRID:AB_2846762.
Fold/Unfold
H1 histone family member 1; H1.1; H1F1; HIST1; HIST1H1A; Histone 1 H1a; Histone cluster 1 H1a;
Immunogens
- Q02539 H11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETVPPAPAASAAPEKPLAGKKAKKPAKAAAASKKKPAGPSVSELIVQAASSSKERGGVSLAALKKALAAAGYDVEKNNSRIKLGIKSLVSKGTLVQTKGTGASGSFKLNKKASSVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPKKAKKPAATRKSSKNPKKPKTVKPKKVAKSPAKAKAVKPKAAKARVTKPKTAKPKKAAPKKK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02539 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
T4 | Phosphorylation | P06493 (CDK1) | Uniprot |
K17 | Ubiquitination | Uniprot | |
K26 | Acetylation | Uniprot | |
K55 | Ubiquitination | Uniprot | |
S61 | Phosphorylation | Uniprot | |
K67 | Sumoylation | Uniprot | |
K67 | Ubiquitination | Uniprot | |
Y74 | Phosphorylation | Uniprot | |
K78 | Acetylation | Uniprot | |
K78 | Ubiquitination | Uniprot | |
S81 | Phosphorylation | Uniprot | |
K88 | Acetylation | Uniprot | |
K88 | Ubiquitination | Uniprot | |
S89 | Phosphorylation | Uniprot | |
S92 | Phosphorylation | Uniprot | |
K93 | Acetylation | Uniprot | |
K93 | Ubiquitination | Uniprot | |
T95 | Phosphorylation | Uniprot | |
K100 | Ubiquitination | Uniprot | |
T102 | Phosphorylation | Uniprot | |
S105 | Phosphorylation | Uniprot | |
S107 | Phosphorylation | Uniprot | |
K109 | Acetylation | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K112 | Ubiquitination | Uniprot | |
K120 | Ubiquitination | Uniprot | |
K125 | Ubiquitination | Uniprot | |
T152 | Phosphorylation | P24941 (CDK2) | Uniprot |
T162 | Phosphorylation | Uniprot | |
S165 | Phosphorylation | Uniprot | |
S166 | Phosphorylation | Uniprot | |
S183 | Phosphorylation | Q15369 (ELOC) , P50750 (CDK9) | Uniprot |
T204 | Phosphorylation | Uniprot |
Research Backgrounds
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.
Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Nucleus. Chromosome.
Note: Mainly localizes in euchromatin.
Interacts with DFFB.
The C-terminal domain is required for high-affinity binding to chromatin.
Belongs to the histone H1/H5 family.
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