GGA3 Antibody - #DF13609

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Product: GGA3 Antibody
Catalog: DF13609
Description: Rabbit polyclonal antibody to GGA3
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 78kDa; 78kD(Calculated).
Uniprot: Q9NZ52
RRID: AB_2846628

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
GGA3 Antibody detects endogenous levels of total GGA3.
RRID:
AB_2846628
Cite Format: Affinity Biosciences Cat# DF13609, RRID:AB_2846628.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor binding protein 3; ADP ribosylation factor binding protein GGA 3; ADP ribosylation factor binding protein GGA3; ADP-ribosylation factor-binding protein GGA3; ARF binding protein GGA 3; ARF binding protein GGA3; ARF-binding protein 3; gamma ear-containing; GGA 3; GGA3; GGA3_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 3; Golgi localized gamma ear containing ARF binding protein 3; Golgi-localized; KIAA0154;

Immunogens

Immunogen:
Uniprot:

Q9NZ52(GGA3_HUMAN) >>Visit HPA database.

Gene(ID):
GGA3(23163)
Expression:
Q9NZ52 GGA3_HUMAN:

Ubiquitously expressed.

Sequence:
MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLLAHKIQSPQEWEALQALTVLEACMKNCGRRFHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKDAYHMLKRQGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQEANKLIKSMVKEDEARIQKVTKRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDILQASDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELDTTNSLSSVLAPAPTPPSSGIPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLLQREQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSLFSTGVAPALAPKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIPTTTPARPLLPFSTGPGSPLFQPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSMLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPLKEKVRLRYKLTFALGEQLSTEVGEVDQFPPVEQWGNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZ52 As Substrate

Site PTM Type Enzyme
K15 Ubiquitination
K38 Ubiquitination
K53 Ubiquitination
S98 Phosphorylation
K100 Ubiquitination
K139 Ubiquitination
T155 Phosphorylation
S159 Phosphorylation
K165 Ubiquitination
K174 Ubiquitination
K176 Ubiquitination
K180 Ubiquitination
K185 Ubiquitination
K195 Ubiquitination
K198 Ubiquitination
K202 Ubiquitination
K210 Ubiquitination
K213 Ubiquitination
T217 Phosphorylation
K258 Ubiquitination
K264 Ubiquitination
S267 Phosphorylation
S275 Phosphorylation
S368 Phosphorylation
S372 Phosphorylation
S425 Phosphorylation
S538 Phosphorylation
R548 Methylation
S559 Phosphorylation
K592 Ubiquitination

Research Backgrounds

Function:

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels intedepently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (By similarity).

PTMs:

Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.

Ubiquitinated.

Proteolytically cleaved during apoptosis by CASP3.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Monomer (Probable). Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs, such as ARF1, ARF5 and ARF6. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR and IGF2R/CI-MPR and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Interacts with ADRA2B. Interacts with NTRK1; the interaction is independent of NTRK1 activation and ubiquitination. Interacts (via VHS domain) with BACE1 (via DXXLL motif).

Family&Domains:

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).

The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.

The unstructured hinge region contains clathrin-binding and an autoinhibitory (DXXLL) motifs.

The GAE domain binds accessory proteins regulating GGAs function.

Belongs to the GGA protein family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

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