Product: OLFM4 Antibody
Catalog: DF13440
Description: Rabbit polyclonal antibody to OLFM4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: Q6UX06
RRID: AB_2846459

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(100%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
OLFM4 Antibody detects endogenous levels of total OLFM4.
RRID:
AB_2846459
Cite Format: Affinity Biosciences Cat# DF13440, RRID:AB_2846459.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antiapoptotic protein GW112; bA209J19.1; G-CSF-stimulated clone 1 protein; GC1; GW112; hGC 1; hGC-1; hOLfD; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLFM4_HUMAN; OLM4; UNQ362;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q6UX06 OLFM4_HUMAN:

Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Sequence:
MRPGLSFLLALLFFLGQAAGDLGDVGPPIPSPGFSSFPGVDSSSSFSSSSRSGSSSSRSLGSGGSVSQLFSNFTGSVDDRGTCQCSVSLPDTTFPVDRVERLEFTAHVLSQKFEKELSKVREYVQLISVYEKKLLNLTVRIDIMEKDTISYTELDFELIKVEVKEMEKLVIQLKESFGGSSEIVDQLEVEIRNMTLLVEKLETLDKNNVLAIRREIVALKTKLKECEASKDQNTPVVHPPPTPGSCGHGGVVNISKPSVVQLNWRGFSYLYGAWGRDYSPQHPNKGLYWVAPLNTDGRLLEYYRLYNTLDDLLLYINARELRITYGQGSGTAVYNNNMYVNMYNTGNIARVNLTTNTIAVTQTLPNAAYNNRFSYANVAWQDIDFAVDENGLWVIYSTEASTGNMVISKLNDTTLQVLNTWYTKQYKPSASNAFMVCGVLYATRTMNTRTEEIFYYYDTNTGKEGKLDIVMHKMQEKVQSINYNPFDQKLYVYNDGYLLNYDLSVLQKPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
83
Sheep
83
Dog
83
Pig
75
Rabbit
75
Chicken
63
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6UX06 As Substrate

Site PTM Type Enzyme
S50 Phosphorylation
S176 Phosphorylation
K200 Acetylation
Y269 Phosphorylation
T308 Phosphorylation
T413 Phosphorylation
T414 Phosphorylation
T423 Phosphorylation

Research Backgrounds

Function:

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

PTMs:

N-glycosylated.

Subcellular Location:

Secreted>Extracellular space. Mitochondrion.
Note: Subcellular location is not clearly defined: has been shown to be secreted (PubMed:16566923), but also in the mitochondrion (PubMed:15059901 and PubMed:20724538), cytoplasm and plasma membrane (PubMed:20724538) and in the nucleus (PubMed:15059901).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues.

Subunit Structure:

Homomultimer; disulfide-linked. Interacts with NDUFA13. Interacts with cell surface lectins (locutions ricinus communis agglutinin I, concanavalin-A and wheat germ agglutinin) and cadherin.

Family&Domains:

The olfactomedin-like domain is involved in the interaction with cadherin.

References

1). Revitalizing gut health: Liangxue guyuan yishen decoction promotes akkermansia muciniphila -induced intestinal stem cell recovery post-radiation in mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39084128) [IF=7.9]

2). Therapeutic mechanism of Liangxue-Guyuan-Yishen decoction on intestinal stem cells and tight junction proteins in gastrointestinal acute radiation syndrome rats. Journal of radiation research, 2023 (PubMed: 37697698) [IF=2.0]

Application: IHC    Species: Rat    Sample:

Fig. 4 Immunohistochemical staining of PCNA and OLFM4 and WB and RT-qPCR to measure relative expression levels of β-catenin, p-β-catenin, C-MYC and WNT-3A. (a, b) Immunohistochemical staining of PCNA in cells at the bottom of the crypt. (c, d) Immunohistochemical staining of OLMF4-positive cells at the bottom of the crypt. Bars represent 100 (10×) and 20 μm (40×), respectively; *P 

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