Product: CAT1 Antibody
Catalog: DF13433
Description: Rabbit polyclonal antibody to CAT1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67kDa; 68kD(Calculated).
Uniprot: P30825
RRID: AB_2846452

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
CAT1 Antibody detects endogenous levels of total CAT1.
RRID:
AB_2846452
Cite Format: Affinity Biosciences Cat# DF13433, RRID:AB_2846452.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amino acid transporter cationic 1; ATRC1; CAT-1; CaT1; CTR1_HUMAN; Ecotropic retroviral leukemia receptor homolog; Ecotropic retroviral receptor; Ecotropic retrovirus receptor homolog; ERR; HCAT1; High affinity cationic amino acid transporter 1; High-affinity cationic amino acid transporter-1; REC1L; Slc7a1; Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1; Solute carrier family 7 member 1; System Y+ basic amino acid transporter;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P30825 SL7A1_HUMAN:

Ubiquitous.

Sequence:
MGCKVLLNIGQQMLRRKVVDCSREETRLSRCLNTFDLVALGVGSTLGAGVYVLAGAVARENAGPAIVISFLIAALASVLAGLCYGEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYIIGTSSVARAWSATFDELIGRPIGEFSRTHMTLNAPGVLAENPDIFAVIIILILTGLLTLGVKESAMVNKIFTCINVLVLGFIMVSGFVKGSVKNWQLTEEDFGNTSGRLCLNNDTKEGKPGVGGFMPFGFSGVLSGAATCFYAFVGFDCIATTGEEVKNPQKAIPVGIVASLLICFIAYFGVSAALTLMMPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Chicken
75
Zebrafish
63
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30825 As Substrate

Site PTM Type Enzyme
K4 Ubiquitination
K215 Ubiquitination
N226 N-Glycosylation
K373 Ubiquitination
Y441 Phosphorylation
K484 Ubiquitination
T620 Phosphorylation
C628 S-Nitrosylation
K629 Sumoylation
K629 Ubiquitination

Research Backgrounds

Function:

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with TM4SF5; the interaction is negatively regulated by arginine.

Family&Domains:

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

References

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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