Product: SV2A Antibody
Catalog: DF13377
Description: Rabbit polyclonal antibody to SV2A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 82kDa; 83kD(Calculated).
Uniprot: Q7L0J3
RRID: AB_2846396

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
SV2A Antibody detects endogenous levels of total SV2A.
RRID:
AB_2846396
Cite Format: Affinity Biosciences Cat# DF13377, RRID:AB_2846396.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0736; OTTHUMP00000014065; SV2; Sv2a; SV2A_HUMAN; Synaptic vesicle glycoprotein 2; Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2a;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPSDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMCLSDSNKGMLGLIVYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSSFVQGYGTFLFCRLLSGVGIGGSIPIVFSYFSEFLAQEKRGEHLSWLCMFWMIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGALTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTIHQEDELIEIQSDTGTWYQRWGVRALSLGGQVWGNFLSCFGPEYRRITLMMMGVWFTMSFSYYGLTVWFPDMIRHLQAVDYASRTKVFPGERVEHVTFNFTLENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCTFINTVFYNTDLFEYKFVNSRLINSTFLHNKEGCPLDVTGTGEGAYMVYFVSFLGTLAVLPGNIVSALLMDKIGRLRMLAGSSVMSCVSCFFLSFGNSESAMIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLGISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
75
Zebrafish
63
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L0J3 As Substrate

Site PTM Type Enzyme
K32 Ubiquitination
Y41 Phosphorylation
S42 Phosphorylation Q6IQ55 (TTBK2)
S45 Phosphorylation Q6IQ55 (TTBK2)
S47 Phosphorylation Q6IQ55 (TTBK2)
S62 Phosphorylation
Y65 Phosphorylation
Y66 Phosphorylation
T71 Phosphorylation
S80 Phosphorylation Q6IQ55 (TTBK2)
S81 Phosphorylation Q6IQ55 (TTBK2)
T84 Phosphorylation Q6IQ55 (TTBK2)
Y94 Phosphorylation
Y98 Phosphorylation
R123 Methylation
S127 Phosphorylation
K143 Ubiquitination
Y212 Phosphorylation
K366 Ubiquitination
K375 Ubiquitination
S393 Phosphorylation
T395 Phosphorylation
S411 Phosphorylation
Y480 Phosphorylation
K485 Ubiquitination
K515 Ubiquitination

Research Backgrounds

Function:

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity).

(Microbial infection) Receptor for the C.botulinum neurotoxin type A2 (BoNT/A, botA); glycosylation is not essential but enhances the interaction. Probably also serves as a receptor for the closely related C.botulinum neurotoxin type A1.

PTMs:

Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.

N-glycosylated.

Subcellular Location:

Cell junction>Synapse>Presynapse. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Multi-pass membrane protein.
Note: Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas (By similarity). Colocalizes with ATP2B1 at photoreceptor synaptic terminals.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2 (By similarity).

(Microbial infection) Interacts with C.botulinum neurotoxin type A2 (BoNT/A, botA). Interaction is improved by glycosylation of SV2.

Family&Domains:

Belongs to the major facilitator superfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

References

1). Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix–Receptor Interaction Pathway. Cancer biotherapy & radiopharmaceuticals, 2021 (PubMed: 34242057) [IF=3.4]

2). RETRACTED: Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix–Receptor Interaction Pathway. Cancer Biotherapy & Radiopharmaceuticals, 2022 (PubMed: 34242057) [IF=3.4]

3). Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix-Receptor Interaction Pathway. Cancer biotherapy & radiopharmaceuticals, 2022 (PubMed: 34242057) [IF=3.4]

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