AP3M1 Antibody - #DF12826

(2)  
Product: AP3M1 Antibody
Catalog: DF12826
Description: Rabbit polyclonal antibody to AP3M1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: Q9Y2T2
RRID: AB_2845787

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
AP3M1 Antibody detects endogenous levels of total AP3M1.
RRID:
AB_2845787
Cite Format: Affinity Biosciences Cat# DF12826, RRID:AB_2845787.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter-related protein complex 3 mu-1 subunit; AP-3 adapter complex mu3A subunit; AP-3 complex subunit mu-1; AP3M1; AP3M1_HUMAN; Clathrin adaptor complex AP3 mu 3A subunit; Mu-adaptin 3A; mu3A; Mu3A-adaptin;

Immunogens

Immunogen:
Uniprot:

Q9Y2T2(AP3M1_HUMAN) >>Visit HPA database.

Gene(ID):
AP3M1(26985)
Sequence:
MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTILRSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACIKLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSISFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITPQKLPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
89
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2T2 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
Y31 Phosphorylation
K135 Ubiquitination
S146 Phosphorylation
K173 Ubiquitination
K283 Methylation
K283 Ubiquitination
K298 Ubiquitination
T304 Phosphorylation
T309 Phosphorylation
T311 Phosphorylation
K345 Ubiquitination
T347 Phosphorylation
K350 Ubiquitination
K355 Ubiquitination
K388 Ubiquitination
K398 Ubiquitination
Y399 Phosphorylation
K406 Ubiquitination
K413 Ubiquitination

Research Backgrounds

Function:

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

Subcellular Location:

Golgi apparatus. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with AGAP1. AP-3 associates with the BLOC-1 complex (By similarity).

(Microbial infection) Interacts with human respiratory virus (HRSV) protein M.

Family&Domains:

Belongs to the adaptor complexes medium subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

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