AP3D1 Antibody - #DF12825

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Product: AP3D1 Antibody
Catalog: DF12825
Description: Rabbit polyclonal antibody to AP3D1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 110 kDa; 130kD(Calculated).
Uniprot: O14617
RRID: AB_2845786

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
AP3D1 Antibody detects endogenous levels of total AP3D1.
RRID:
AB_2845786
Cite Format: Affinity Biosciences Cat# DF12825, RRID:AB_2845786.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter-related protein complex 3 subunit delta-1; adaptor-related protein complex 3, delta 1 subunit; ADTD; AP 3 complex delta subunit; AP-3 complex subunit delta; AP-3 complex subunit delta-1; ap3d1; AP3D1_HUMAN; Delta 1 subunit of adaptor-related protein complex 3; Delta Adaptin; Delta-adaptin; hBLVR; Subunit of putative vesicle coat adaptor complex AP 3;

Immunogens

Immunogen:
Uniprot:

O14617(AP3D1_HUMAN) >>Visit HPA database.

Gene(ID):
AP3D1(8943)
Expression:
O14617 AP3D1_HUMAN:

Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.

Sequence:
MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVCKLTYLQMLGYDISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDLSSPSQYDTGVALTGLSCFVTPDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPESLRPAFPRLKEKLEDPDPGVQSAAVNVICELARRNPKNYLSLAPLFFKLMTSSTNNWVLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECVNTVIAVLISLSSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLDDKDESIRLRALDLLYGMVSKKNLMEIVKKLMTHVDKAEGTTYRDELLTKIIDICSQSNYQYITNFEWYISILVELTRLEGTRHGHLIAAQMLDVAIRVKAIRKFAVSQMSALLDSAHLLASSTQRNGICEVLYAAAWICGEFSEHLQEPHHTLEAMLRPRVTTLPGHIQAVYVQNVVKLYASILQQKEQAGEAEGAQAVTQLMVDRLPQFVQSADLEVQERASCILQLVKHIQKLQAKDVPVAEEVSALFAGELNPVAPKAQKKVPVPEGLDLDAWINEPLSDSESEDERPRAVFHEEEQRRPKHRPSEADEEELARRREARKQEQANNPFYIKSSPSPQKRYQDTPGVEHIPVVQIDLSVPLKVPGLPMSDQYVKLEEERRHRQKLEKDKRRKKRKEKEKKGKRRHSSLPTESDEDIAPAQQVDIVTEEMPENALPSDEDDKDPNDPYRALDIDLDKPLADSEKLPIQKHRNTETSKSPEKDVPMVEKKSKKPKKKEKKHKEKERDKEKKKEKEKKKSPKPKKKKHRKEKEERTKGKKKSKKQPPGSEEAAGEPVQNGAPEEEQLPPESSYSLLAENSYVKMTCDIRGSLQEDSQVTVAIVLENRSSSILKGMELSVLDSLNARMARPQGSSVHDGVPVPFQLPPGVSNEAQYVFTIQSIVMAQKLKGTLSFIAKNDEGATHEKLDFRLHFSCSSYLITTPCYSDAFAKLLESGDLSMSSIKVDGIRMSFQNLLAKICFHHHFSVVERVDSCASMYSRSIQGHHVCLLVKKGENSVSVDGKCSDSTLLSNLLEEMKATLAKC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14617 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K4 Ubiquitination
K7 Ubiquitination
S9 Phosphorylation
K16 Ubiquitination
K34 Ubiquitination
K44 Ubiquitination
K55 Ubiquitination
K164 Ubiquitination
K165 Ubiquitination
K176 Acetylation
K176 Ubiquitination
S180 Phosphorylation
K191 Ubiquitination
T270 Phosphorylation
K332 Ubiquitination
K336 Ubiquitination
K342 Ubiquitination
K352 Ubiquitination
S355 Phosphorylation
Y365 Phosphorylation
K379 Ubiquitination
K386 Acetylation
K584 Ubiquitination
K588 Ubiquitination
K610 Ubiquitination
K613 Ubiquitination
S632 Phosphorylation
S634 Phosphorylation
S636 Phosphorylation
S658 Phosphorylation
K673 Ubiquitination
Y682 Phosphorylation
S685 Phosphorylation
S686 Phosphorylation
S688 Phosphorylation
T696 Phosphorylation
S721 Phosphorylation
Y724 Phosphorylation
K736 Acetylation
S758 Phosphorylation
S759 Phosphorylation
T762 Phosphorylation
S764 Phosphorylation
T778 Phosphorylation
S788 Phosphorylation
Y799 Phosphorylation
K808 Ubiquitination
K820 Ubiquitination
T824 Phosphorylation
T826 Phosphorylation
S827 Phosphorylation
S829 Phosphorylation
S869 Phosphorylation
S1070 Phosphorylation
S1071 Phosphorylation
C1103 S-Nitrosylation
K1132 Ubiquitination

Research Backgrounds

Function:

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T-cell and NK cell degranulation. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals (By similarity).

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.

Subunit Structure:

AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2) (By similarity). Interacts with SLC30A2.

Family&Domains:

Belongs to the adaptor complexes large subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

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