SPRR3 Antibody - #DF12751
(1)
(4)
Product: | SPRR3 Antibody |
Catalog: | DF12751 |
Description: | Rabbit polyclonal antibody to SPRR3 |
Application: | WB IF/ICC |
Reactivity: | Human, Rat |
Mol.Wt.: | 25 kDa; 18kD(Calculated). |
Uniprot: | Q9UBC9 |
RRID: | AB_2845712 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
SPRR3 Antibody detects endogenous levels of total SPRR3.
RRID:
AB_2845712
Cite Format: Affinity Biosciences Cat# DF12751, RRID:AB_2845712.
Cite Format: Affinity Biosciences Cat# DF12751, RRID:AB_2845712.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
22 kDa pancornulin; Cornifin beta; Esophagin; Small proline rich protein 3; Small proline-rich protein 3; SPRC; Sprr3; SPRR3_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9UBC9 SPRR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK
PTMs - Q9UBC9 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
T11 | Phosphorylation | Uniprot | |
Y106 | Phosphorylation | Uniprot | |
Y146 | Phosphorylation | Uniprot | |
T147 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Cross-linked envelope protein of keratinocytes.
Subcellular Location:
Cytoplasm.
Family&Domains:
Belongs to the cornifin (SPRR) family.
References
1). SPRR3, a novel miR‑338‑3p target, regulates the malignant progression of clear cell renal cell carcinoma in vitro via the PI3K/Akt signaling pathway. Experimental and Therapeutic Medicine, 2022
(PubMed: 35350667)
[IF=2.7]
Application: WB Species: Human Sample: ccRCC cells
Figure 1
Association between SPRR3 expression and the prognosis of ccRCC, and detection of SPRR3 expression in human normal renal cells HK-2 and ccRCC cell lines. (A) Survival analysis of 531 patients with ccRCC by Kaplan-Meier curve (P=0.0017). SPRR3 expression in HK-2, 786-O, CaKi-1 and UMRC-2 cell lines, as detected using (B) reverse transcription-quantitative PCR and (C) western blotting. **P<0.01 vs. HK-2. SPRR3, small proline-rich repeat protein 3; ccRCC, clear cell renal cell carcinoma.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.