TEX10 Antibody | Affinity Biosciences

WB
IHC
Cites

1/3

Western blot analysis of extracts from HeLa cells, using TEX10 antibody.

DF12486 at 1/100 staining Mouse liver tissue by IHC-P.

Figure 1 Screening indicates that TEX10 is upregulated and is associated with prognosis in CRC.

Product:	TEX10 Antibody
Catalog:	DF12486
Description:	Rabbit polyclonal antibody to TEX10
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	95-100 kDa; 106kD(Calculated).
Uniprot:	Q9NXF1
RRID:	AB_2845291

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TEX10 Antibody detects endogenous levels of total TEX10.

RRID:

AB_2845291
Cite Format: Affinity Biosciences Cat# DF12486, RRID:AB_2845291.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q9NXF1(TEX10_HUMAN) >>Visit HPA database.

Gene(ID):

TEX10(54881)

Sequence:

Q9NXF1 TEX10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTKKRKRQHDFQKVKLKVGKKKPKLQNATPTNFKTKTIHLPEQLKEDGTLPTNNRKLNIKDLLSQMHHYNAGVKQSALLGLKDLLSQYPFIIDAHLSNILSEVTAVFTDKDANVRLAAVQLLQFLAPKIRAEQISPFFPLVSAHLSSAMTHITEGIQEDSLKVLDILLEQYPALITGRSSILLKNFVELISHQQLSKGLINRDRSQSWILSVNPNRRLTSQQWRLKVLVRLSKFLQALADGSSRLRESEGLQEQKENPHATSNSIFINWKEHANDQQHIQVYENGGSQPNVSSQFRLRYLVGGLSGVDEGLSSTENLKGFIEIIIPLLIECWVEAVPPQLATPVGNGIEREPLQVMQQVLNIISLLWKLSKQQDETHKLESWLRKNYLIDFKHHFMSRFPYVLKEITKHKRKEPNKSIKHCTVLSNNIDHLLLNLTLSDIMVSLANASTLQKDCSWIEMIRKFVTETLEDGSRLNSKQLNRLLGVSWRLMQIQPNREDTETLIKAVYTLYQQRGLILPVRTLLLKFFSKIYQTEELRSCRFRYRSKVLSRWLAGLPLQLAHLGSRNPELSTQLIDIIHTAAARANKELLKSLQATALRIYDPQEGAVVVLPADSQQRLVQLVYFLPSLPADLLSRLSRCCIMGRLSSSLAAMLIGILHMRSSFSGWKYSAKDWLMSDVDYFSFLFSTLTGFSKEELTWLQSLRGVPHVIQTQLSPVLLYLTDLDQFLHHWDVTEAVFHSLLVIPARSQNFDILQSAISKHLVGLTVIPDSTAGCVFGVICKLLDHTCVVSETLLPFLASCCYSLLYFLLTIEKGEAEHLRKRDKLWGVCVSILALLPRVLRLMLQSLRVNRVGPEELPVVGQLLRLLLQHAPLRTHMLTNAILVQQIIKNITTLKSGSVQEQWLTDLHYCFNVYITGHPQGPSALATVY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NXF1 As Substrate

Q9NXF1

Site	PTM Type	Source
T29	Phosphorylation	Uniprot
T31	Phosphorylation	Uniprot
K34	Methylation	Uniprot
K34	Ubiquitination	Uniprot
K36	Methylation	Uniprot
K45	Ubiquitination	Uniprot
T49	Phosphorylation	Uniprot
T52	Phosphorylation	Uniprot
S76	Phosphorylation	Uniprot
S196	Phosphorylation	Uniprot
K197	Ubiquitination	Uniprot
S305	Phosphorylation	Uniprot
S312	Phosphorylation	Uniprot
K378	Ubiquitination	Uniprot
K462	Ubiquitination	Uniprot
K529	Acetylation	Uniprot
K590	Ubiquitination	Uniprot
S846	Phosphorylation	Uniprot
K889	Acetylation	Uniprot

Research Backgrounds

Q9NXF1_TEX10_HUMAN

Function:

Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit.

Subcellular Location:

Nucleus>Nucleoplasm. Cytoplasm. Nucleus>Nucleolus.
Note: Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions.

Subunit Structure:

Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Component of the PELP1 complex, composed of at least PELP1, TEX10 and WDR18. The complex interacts with pre-60S ribosome particles.

Family&Domains:

Belongs to the IPI1/TEX10 family.

References

1). RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation. Advanced Science, 2020 (PubMed: 32995120) [IF=15.1]

Application: WB Species: Human Sample: CRC tissues

Figure 1 Screening indicates that TEX10 is upregulated and is associated with prognosis in CRC. A) Schematic of the shRNA library screening strategy for genes that affected CRC cell proliferation. B) Schematic of the overlapping results of shRNA‐screened genes and differentially expressed genes in tumors. C) Heatmap of the overlapping genes in (B). D) TEX10 expression profile in various tumor types and normal tissues as identified through the GEPIA website. E) qPCR analysis of TEX10 expression in 16 CRC tissues and adjacent normal mucosal tissues. F) IB analysis of TEX10 protein expression in paired CRC tissues. N, normal tissues; T, tumor tissues. G) IHC scores of TEX10 in 129 paired CRC and adjacent normal tissues. H) Kaplan–Meier analysis of overall survival and I) progression‐free survival according to TEX10 levels in CRC patients. *P < 0.05, **P < 0.01, and ***P < 0.001 (E,G) two‐tailed Student's t‐test or H,I) log‐rank test).

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TEX10 Antibody - #DF12486