PMPCB Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat liver tissue, using PMPCB antibody.

Western blot analysis of extracts from ratbrain, using PMPCB antibody.

Product:	PMPCB Antibody
Catalog:	DF12305
Description:	Rabbit polyclonal antibody to PMPCB
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	43-52 kDa; 54kD(Calculated).
Uniprot:	O75439
RRID:	AB_2845110

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

PMPCB Antibody detects endogenous levels of total PMPCB.

RRID:

AB_2845110
Cite Format: Affinity Biosciences Cat# DF12305, RRID:AB_2845110.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta MPP; Beta-MPP; BetaMPP; Mitochondrial processing peptidase subunit beta; Mitochondrial-processing peptidase subunit beta; MPP11; MPPB; MPPB_HUMAN; MPPP52; P 52; P-52; P52; Peptidase mitichondrial processing beta; PMPCB;

Immunogens

Immunogen:

Uniprot:

O75439(MPPB_HUMAN) >>Visit HPA database.

Gene(ID):

PMPCB(9512)

Sequence:

O75439 MPPB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAAARVVLSSAARRRLWGFSESLLIRGAAGRSLYFGENRLRSTQAATQVVLNVPETRVTCLESGLRVASEDSGLSTCTVGLWIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSTLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQNTALGRTILGPTENIKSISRKDLVDYITTHYKGPRIVLAAAGGVSHDELLDLAKFHFGDSLCTHKGEIPALPPCKFTGSEIRVRDDKMPLAHLAIAVEAVGWAHPDTICLMVANTLIGNWDRSFGGGMNLSSKLAQLTCHGNLCHSFQSFNTSYTDTGLWGLYMVCESSTVADMLHVVQKEWMRLCTSVTESEVARARNLLKTNMLLQLDGSTPICEDIGRQMLCYNRRIPIPELEARIDAVNAETIREVCTKYIYNRSPAIAAVGPIKQLPDFKQIRSNMCWLRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75439 As Substrate

O75439

Site	PTM Type	Source
S11	Phosphorylation	Uniprot
S12	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
T45	Phosphorylation	Uniprot
T49	Phosphorylation	Uniprot
T58	Phosphorylation	Uniprot
C62	S-Nitrosylation	Uniprot
K95	Ubiquitination	Uniprot
Y133	Phosphorylation	Uniprot
Y141	Phosphorylation	Uniprot
Y142	Phosphorylation	Uniprot
K148	Ubiquitination	Uniprot
T205	Phosphorylation	Uniprot
K219	Ubiquitination	Uniprot
K224	Ubiquitination	Uniprot
Y229	Phosphorylation	Uniprot
K235	Ubiquitination	Uniprot
C419	S-Nitrosylation	Uniprot
K472	Ubiquitination	Uniprot
K478	Ubiquitination	Uniprot

Research Backgrounds

O75439_MPPB_HUMAN

Function:

Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (Probable). Preferentially, cleaves after an arginine at position P2 (By similarity). Required for PINK1 turnover by coupling PINK1 mitochondrial import and cleavage, which results in subsequent PINK1 proteolysis.

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits, forming the mitochondrial processing protease (MPP) in which PMPCA is involved in substrate recognition and binding and PMPCB is the catalytic subunit.

Family&Domains:

Belongs to the peptidase M16 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PMPCB Antibody - #DF12305