CHCHD6 Antibody - #DF12255

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Product: CHCHD6 Antibody
Catalog: DF12255
Description: Rabbit polyclonal antibody to CHCHD6
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Rabbit
Mol.Wt.: 26-29 kDa; 26kD(Calculated).
Uniprot: Q9BRQ6
RRID: AB_2845060

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(89%), Bovine(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
CHCHD6 Antibody detects endogenous levels of total CHCHD6.
RRID:
AB_2845060
Cite Format: Affinity Biosciences Cat# DF12255, RRID:AB_2845060.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CHCH6_HUMAN; Chchd6; CHCM1; Coiled coil helix cristae morphology 1; Coiled coil helix cristae morphology protein 1; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial; Mic25; MICOS complex subunit MIC25; PPP1R23; Protein phosphatase 1 regulatory subunit 23;

Immunogens

Immunogen:
Uniprot:

Q9BRQ6(MIC25_HUMAN) >>Visit HPA database.

Gene(ID):
CHCHD6(84303)
Sequence:
MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
100
Pig
89
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRQ6 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
S42 Phosphorylation
S43 Phosphorylation
T49 Phosphorylation
K65 Ubiquitination
S67 Phosphorylation
S74 Phosphorylation
K84 Acetylation
K100 Acetylation
K106 Acetylation
K114 Acetylation
K114 Ubiquitination
K117 Acetylation
K133 Acetylation
S134 Phosphorylation
T153 Phosphorylation
Y155 Phosphorylation
K156 Ubiquitination
Y170 Phosphorylation
S173 Phosphorylation
S186 Phosphorylation
T187 Phosphorylation

Research Backgrounds

Function:

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.

Subcellular Location:

Mitochondrion inner membrane>Lipid-anchor. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with DISC1. Interacts with DISC1. Interacts with IMMT/MIC60.

Family&Domains:

Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic25 subfamily.

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