Product: Phospho-LRP5 (Tyr1473) Antibody
Catalog: AF4345
Description: Rabbit polyclonal antibody to Phospho-LRP5 (Tyr1473)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 200kDa; 179kD(Calculated).
Uniprot: O75197
RRID: AB_2844424

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-LRP5 (Tyr1473) Antibody detects endogenous levels of LRP5 only when phosphorylated at Tyr1473.
RRID:
AB_2844424
Cite Format: Affinity Biosciences Cat# AF4345, RRID:AB_2844424.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMND1; EVR1; EVR4; HBM; Low density lipoprotein receptor related protein 5; Low density lipoprotein receptor related protein 7; Low-density lipoprotein receptor-related protein 5; LR3; LRP-5; Lrp5; LRP5_HUMAN; LRP7; OPPG; OPS; OPTA1; Osteoporosis pseudoglioma syndrome; VBCH2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O75197 LRP5_HUMAN:

Widely expressed, with the highest level of expression in the liver and in aorta.

Sequence:
MEAAPPGPPWPLLLLLLLLLALCGCPAPAAASPLLLFANRRDVRLVDAGGVKLESTIVVSGLEDAAAVDFQFSKGAVYWTDVSEEAIKQTYLNQTGAAVQNVVISGLVSPDGLACDWVGKKLYWTDSETNRIEVANLNGTSRKVLFWQDLDQPRAIALDPAHGYMYWTDWGETPRIERAGMDGSTRKIIVDSDIYWPNGLTIDLEEQKLYWADAKLSFIHRANLDGSFRQKVVEGSLTHPFALTLSGDTLYWTDWQTRSIHACNKRTGGKRKEILSALYSPMDIQVLSQERQPFFHTRCEEDNGGCSHLCLLSPSEPFYTCACPTGVQLQDNGRTCKAGAEEVLLLARRTDLRRISLDTPDFTDIVLQVDDIRHAIAIDYDPLEGYVYWTDDEVRAIRRAYLDGSGAQTLVNTEINDPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTSRKILVSEDLDEPRAIALHPVMGLMYWTDWGENPKIECANLDGQERRVLVNASLGWPNGLALDLQEGKLYWGDAKTDKIEVINVDGTKRRTLLEDKLPHIFGFTLLGDFIYWTDWQRRSIERVHKVKASRDVIIDQLPDLMGLKAVNVAKVVGTNPCADRNGGCSHLCFFTPHATRCGCPIGLELLSDMKTCIVPEAFLVFTSRAAIHRISLETNNNDVAIPLTGVKEASALDFDVSNNHIYWTDVSLKTISRAFMNGSSVEHVVEFGLDYPEGMAVDWMGKNLYWADTGTNRIEVARLDGQFRQVLVWRDLDNPRSLALDPTKGYIYWTEWGGKPRIVRAFMDGTNCMTLVDKVGRANDLTIDYADQRLYWTDLDTNMIESSNMLGQERVVIADDLPHPFGLTQYSDYIYWTDWNLHSIERADKTSGRNRTLIQGHLDFVMDILVFHSSRQDGLNDCMHNNGQCGQLCLAIPGGHRCGCASHYTLDPSSRNCSPPTTFLLFSQKSAISRMIPDDQHSPDLILPLHGLRNVKAIDYDPLDKFIYWVDGRQNIKRAKDDGTQPFVLTSLSQGQNPDRQPHDLSIDIYSRTLFWTCEATNTINVHRLSGEAMGVVLRGDRDKPRAIVVNAERGYLYFTNMQDRAAKIERAALDGTEREVLFTTGLIRPVALVVDNTLGKLFWVDADLKRIESCDLSGANRLTLEDANIVQPLGLTILGKHLYWIDRQQQMIERVEKTTGDKRTRIQGRVAHLTGIHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNLLTCGEPPTCSPDQFACATGEIDCIPGAWRCDGFPECDDQSDEEGCPVCSAAQFPCARGQCVDLRLRCDGEADCQDRSDEADCDAICLPNQFRCASGQCVLIKQQCDSFPDCIDGSDELMCEITKPPSDDSPAHSSAIGPVIGIILSLFVMGGVYFVCQRVVCQRYAGANGPFPHEYVSGTPHVPLNFIAPGGSQHGPFTGIACGKSMMSSVSLMGGRGGVPLYDRNHVTGASSSSSSSTKATLYPPILNPPPSPATDPSLYNMDMFYSSNIPATARPYRPYIIRGMAPPTTPCSTDVCDSDYSASRWKASKYYLDLNSDSDPYPPPPTPHSQYLSAEDSCPPSPATERSYFHLFPPPPSPCTDSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
88
Horse
0
Bovine
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75197 As Substrate

Site PTM Type Enzyme
K121 Ubiquitination
K143 Ubiquitination
T185 Phosphorylation
K592 Ubiquitination
K802 Ubiquitination
K980 Ubiquitination
S1138 Phosphorylation
S1142 Phosphorylation
K1231 Ubiquitination
Y1473 Phosphorylation
T1479 Phosphorylation
T1492 Phosphorylation
Y1494 Phosphorylation
S1503 Phosphorylation
Y1528 Phosphorylation
Y1531 Phosphorylation
Y1552 Phosphorylation
S1589 Phosphorylation
S1593 Phosphorylation
S1615 Phosphorylation

Research Backgrounds

Function:

Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins. Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration. In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation (By similarity). During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass. Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs. Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development. Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis (By similarity).

PTMs:

Phosphorylation of cytoplasmic PPPSP motifs regulates the signal transduction of the Wnt signaling pathway through acting as a docking site for AXIN1.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Endoplasmic reticulum.
Note: Chaperoned to the plasma membrane by MESD.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with the highest level of expression in the liver and in aorta.

Subunit Structure:

Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling (By similarity). Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts with FZD8; the interaction is formed on WNT-binding and signaling. Interacts (via the phosphorylated PPPSP motif domains) with AXIN1; the interaction prevents inhibition of beta-catenin phosphorylation and signaling and is enhanced in the presence of GSK3B and WNT1 or WNT3A. Interacts (via beta-propeller regions 3 and 4) with DKK1; the interaction, enhanced by MESD and/or KREMEN, inhibits beta-catenin signaling by preventing GSK3-mediated phosphorylation of the PPPSP motifs and subsequent, AXIN1 binding. Interacts with MESD; the interaction prevents the formation of LRP5 aggregates, targets LRP5 to the plasma membrane and, when complexed with KREMEN2, increases DKK1 binding. Interacts with CSNK1E. Interacts with SOST; the interaction antagonizes canonical Wnt signaling. Interacts with APCDD1. Interacts with CAPRIN2.

Family&Domains:

Belongs to the LDLR family.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). Sini San Inhibits Chronic Psychological Stress-Induced Breast Cancer Stemness by Suppressing Cortisol-Mediated GRP78 Activation. Frontiers in Pharmacology, 2021 (PubMed: 34912211) [IF=5.6]

Application: WB    Species: Mouse    Sample: 4T1 cells

FIGURE 6 SNS interrupts the interaction between GRP78 and LRP5 to suppress Wnt/β-catenin signaling. (A) After 4T1 cells were treated with cortisol for 24 h, the translocation of GRP78 to the cell membrane was monitored by immunofluorescence. Red: DiI-cell membrane tracker; green: GRP78. Co-localization of GRP78 and the cell membrane is shown as yellow fluorescence. (B) The 4T1 cells were treated with cortisol for 24 h, and the co-localization of GRP78 and LRP5 was detected by immunofluorescence. Co-localization is shown as yellow fluorescence. (C) The effect of SNS (200 μg/ml) on cortisol-induced cell membrane translocation of GRP78 was detected by immunofluorescence following SNS treatment for 24 h. (D) The effect of SNS (200 μg/ml) on the co-localization of GRP78 and LRP5 induced by cortisol was detected by immunofluorescence following SNS treatment for 24 h. (E) The expression of LRP5, p-LRP5, and β-catenin in the GRP78-overexpressing 4T1 cells was measured by western blots. (F) The 4T1 cells or GRP78-overexpressing 4T1 cells were treated with SNS (200 μg/ml) for 24 h, and the changes in cortisol-induced LRP5, p-LRP5, and β-catenin expression were detected by western blots. (G) The 4T1 cells were treated with SNS (200 μg/ml) for 24 h, and changes in the interaction of GRP78 with LRP5 were analyzed by Co-IP assays. Input represents the total protein extracts prepared without the antibody coupling resin. NC indicates the negative control prepared by adding quenching buffer to the antibody coupling resin. NS, not significant. The scale bars indicate 10 μm. One representative experiment of three independent experiments is displayed.

2). Raddeanin A Protects the BRB Through Inhibiting Inflammation and Apoptosis in the Retina of Alzheimer's Disease. Neurochemical research, 2024 (PubMed: 38834846) [IF=4.4]

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