Product: IL-2RA/CD25 Antibody
Catalog: AF7675
Description: Rabbit polyclonal antibody to IL-2RA/CD25
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Horse, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: P01589
RRID: AB_2844039

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 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(89%), Dog(86%)
Clonality:
Polyclonal
Specificity:
IL-2RA/CD25 Antibody detects endogenous levels of total IL-2RA/CD25.
RRID:
AB_2844039
Cite Format: Affinity Biosciences Cat# AF7675, RRID:AB_2844039.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Interleukin 2 receptor alpha chain; CD25; CD25 antigen; IDDM10; IL 2 receptor alpha subunit; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2 RA; IL2-RA; IL2R; IL2R, alpha chain; IL2RA; IL2RA_HUMAN; IMD41; Interleukin 2 receptor alpha; Interleukin 2 receptor; Interleukin-2 receptor subunit alpha; p55; t-cell growth factor receptor; TAC; TAC antigen; TCGFR;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDSYLLMWGLLTFIMVPGCQAELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCLVTTTDFQIQTEMAATMETSIFTTEYQVAVAGCVFLLISVLLLSGLTWQRRQRKSRRTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
89
Dog
86
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01589 As Substrate

Site PTM Type Enzyme
N70 N-Glycosylation
N89 N-Glycosylation
T218 O-Glycosylation
T224 O-Glycosylation
T229 O-Glycosylation
T237 O-Glycosylation
S268 Phosphorylation P17252 (PRKCA)
T271 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

References

1). Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance. Journal of Translational Medicine, 2021 (PubMed: 34407839) [IF=7.4]

Application: WB    Species: Mouse    Sample: colon tissue

Fig. 6 L.p R3 attenuates colonic inflammatory responses but induces Treg responses in DSS-induced experimental murine colitis. A The expression of Foxp3, IL-10, IL-17A and IFN-γ were detected in the colon by the immunohistochemical method. B Pathological score of Foxp3, IL-10, IL-17A and IFN-γ expression in colon tissue. C Evaluate the expression of CD4, CD25, Foxp3, IL-17A, IFN-γ, IL-10 in colon tissue by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001

2). Salvianolic acid B alleviates autoimmunity in Treg-deficient mice via inhibiting IL2-STAT5 signaling. Phytotherapy research : PTR, 2024 (PubMed: 38887974) [IF=7.2]

3). SLC35A2 expression drives breast cancer progression via ERK pathway activation. The FEBS journal, 2023 (PubMed: 38143314) [IF=5.4]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.