Product: DIO3 Antibody
Catalog: DF8532
Description: Rabbit polyclonal antibody to DIO3
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 31 kDa; 34kD(Calculated).
Uniprot: P55073
RRID: AB_2841736

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DIO3 Antibody detects endogenous levels of total DIO3.
RRID:
AB_2841736
Cite Format: Affinity Biosciences Cat# DF8532, RRID:AB_2841736.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5DIII; D3; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; Dio3; DIOIII; IOD3_HUMAN; Iodothyronine deiodinase placental type; MGC124117; MGC124118; Thyroxine deiodinase type III; Thyroxine deiodinase type III (selenoprotein); TXDI3; Type 3 DI; Type 3 iodothyronine selenodeiodinase; Type III 5' deiodinase; Type III iodothyronine deiodinase; Type-III 5''-deiodinase;

Immunogens

Immunogen:

A synthesized peptide derived from human DIO3, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
P55073 IOD3_HUMAN:

Expressed in placenta and several fetal tissues.

Sequence:
MPRQATSRLVVGEGEGSQGASGPAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGNRPLVLNFGSCTUPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGARPRRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
75
Xenopus
67
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55073 As Substrate

Site PTM Type Enzyme
S252 Phosphorylation

Research Backgrounds

Function:

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in placenta and several fetal tissues.

Subunit Structure:

Homodimer. May undergo minor heretodimerization with DIO1 and DIO2.

Family&Domains:

Belongs to the iodothyronine deiodinase family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). Dlk1-Dio3 Locus-Derived lncRNA Dio3os Affects Growth, Development and Reproductive Activity in Mice. , 2023

Application: WB    Species: Mouse    Sample: ESCs

Fig. 2: (A) Effects of Dio3os on Dlk1, Gtl2 and Dio3 in mouse ESCs. (B) Effects of Dio3os on DIO3 protein in mouse ESCs. (C) Relative mRNA expression levels of Dio3os and Dio3 in different mouse tissues.

Application: WB    Species: Mouse    Sample: ESCs

Fig. 2: (A) Effects of Dio3os on Dlk1, Gtl2 and Dio3 in mouse ESCs. (B) Effects of Dio3os on DIO3 protein in mouse ESCs. (C) Relative mRNA expression levels of Dio3os and Dio3 in different mouse tissues.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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